Immunohistochemical identification of multipotent adult progenitor cells from human bone marrow after transplantation into the rat brain

Li Ru Zhao, Wei Ming Duan, Morayma Reyes, Catherine M. Verfaillie, Walter C. Low

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

In the immunohistochemical analysis of the brain, tissue preparation and fixation are critical steps. During our studies of transplanting human bone marrow multipotent adult progenitor cells (hMAPCs) into the rat brain, we noticed that various methods of brain tissue preparation and fixation differentially influenced antigenic preservation in the transplants and in the host brain. Here, we report a simple, effective and reproducible method of tissue preparation and fixation that results in the immunohistochemical labeling of transplanted and host cells.

Original languageEnglish (US)
Pages (from-to)38-45
Number of pages8
JournalBrain Research Protocols
Volume11
Issue number1
DOIs
StatePublished - Mar 2003

Keywords

  • Bone marrow stem cell
  • Ethanol
  • Fast frozen
  • Formaldehyde
  • Transplantation

ASJC Scopus subject areas

  • Neuroscience(all)

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