Immunohistochemical evaluation of heat shock proteins in normal and preinvasive lesions of the cervix

Philip E. Castle, Raheela Ashfaq, Faryal Ansari, Carolyn Y. Muller

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Heat Shock Proteins (HSPs) are molecular chaperons with multiple functions relating to cellular homeostasis. Primary, HSPs are expressed in response to cellular stresses that may also include carcinogenesis. Patterns of expression have not been extensively evaluated in the multi-step carcinogenesis of cervical cancer (Normal → HPV infection → Cervical Precancer → Cancer). We evaluated the expression of HSP40, HSP60, HSP70, and HSP90 in normal tissues (N=30), in cervical intraepithelial neoplasia grade 1 (CIN1)(synonymous with productive HPV infections) (N=32), and in CIN3 (cervical precancer)(N=25) by immunohistochemistry staining (graded 0-3) and compared the results to p16 INK4a, a biomarker of oncogenic HPV infections and CIN3. We found strong patterns of increased HSP40, HSP60, and HSP70 immunostaining with increasing severity of the lesion in a manner similar to p16INK4a (PTrend<0.0005). No difference in staining intensity by grade of lesion was observed for HSP90 (PTrend=0.8). Tissue patterns in CIN3 of diffuse immunostaining for HSP40 and HSP70 were analogous to those observed for p16INK4a; HSP60 immunostaining appeared more punctate within cells than for other antigens although similar tissue patterns were observed. We conclude that HSP40, HSP60, and HSP70 expressions are up-regulated in response to the development of CIN3 akin to p16INK4a expression.

Original languageEnglish (US)
Pages (from-to)245-252
Number of pages8
JournalCancer Letters
Volume229
Issue number2
DOIs
StatePublished - Nov 18 2005
Externally publishedYes

Keywords

  • Cervical intraepithelial neoplasia
  • HSP40
  • HSP60
  • HSP70
  • HSP90
  • Heat shock proteins
  • Human papillomavirus (HPV)
  • Immunohistochemistry

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Immunohistochemical evaluation of heat shock proteins in normal and preinvasive lesions of the cervix'. Together they form a unique fingerprint.

  • Cite this