Immunohistochemical detection of myelin basic protein is a sensitive marker of myelination in second trimester human fetal spinal cord

Surender R. Bodhireddy, William D. Lyman, William K. Rashbaum, Karen M. Weidenheim

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The Luxol fast blue (LFB) technique is widely used for the assessment of myelination. Lectin histochemistry using peanut agglutinin (PNA) has also been employed for this purpose. Recently, immunohistochemical methods using antibodies to several myelin-related proteins have been adopted to study myelination in humans. However, the relative sensitivities of these different methods for the detection of early myelination in the human fetal central nervous system have not been determined. Vibratome sections of cervical spinal cord from 15 human abortuses ranging in age from 15 to 24 gestational weeks (GW) were probed with immunohistochemical methods using antibodies to myelin basic protein (MBP), 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), and myelin-associated glycoprotein (MAG). In addition, LFB and PNA histochemistry was employed. The degree of myelination observed in immunohistochemically stained sections was compared to that found in corresponding LFB- and PNA-stained paraffin-embedded tissues. The intensity of myelination was graded by two observers on a scale of 0 (none), +1 (mild), +2 (moderate), and +3 (marked). At all ages examined, the MBP immunohistochemical method revealed more myelin than LFB or MAG staining. CNPase could not be reliably detected until after 18 GW. Peanut agglutinin stained myelin, but subpial astrocytes and the intervening neuropil were also stained. These results suggest that MBP is a more sensitive marker for early human fetal myelination than CNPase, MAG, PNA or LFB.

Original languageEnglish (US)
Pages (from-to)144-149
Number of pages6
JournalJournal of Neuropathology and Experimental Neurology
Volume53
Issue number2
StatePublished - 1994

Fingerprint

Peanut Agglutinin
Myelin Basic Protein
Second Pregnancy Trimester
Myelin-Associated Glycoprotein
Spinal Cord
Cyclic Nucleotides
Phosphoric Diester Hydrolases
Myelin Sheath
2',3'-Cyclic Nucleotide 3'-Phosphodiesterase
Myelin Proteins
Neuropil
Antibodies
Lectins
Astrocytes
Paraffin
Central Nervous System
Luxol Fast Blue MBS
Staining and Labeling

Keywords

  • Central nervous system
  • Immunohistochemistry
  • Myelin proteins
  • Spinal cord

ASJC Scopus subject areas

  • Clinical Neurology
  • Pathology and Forensic Medicine
  • Cellular and Molecular Neuroscience
  • Neurology
  • Neuroscience(all)

Cite this

Immunohistochemical detection of myelin basic protein is a sensitive marker of myelination in second trimester human fetal spinal cord. / Bodhireddy, Surender R.; Lyman, William D.; Rashbaum, William K.; Weidenheim, Karen M.

In: Journal of Neuropathology and Experimental Neurology, Vol. 53, No. 2, 1994, p. 144-149.

Research output: Contribution to journalArticle

Bodhireddy, Surender R. ; Lyman, William D. ; Rashbaum, William K. ; Weidenheim, Karen M. / Immunohistochemical detection of myelin basic protein is a sensitive marker of myelination in second trimester human fetal spinal cord. In: Journal of Neuropathology and Experimental Neurology. 1994 ; Vol. 53, No. 2. pp. 144-149.
@article{c4345336d96b413eb218ed8f807f6e00,
title = "Immunohistochemical detection of myelin basic protein is a sensitive marker of myelination in second trimester human fetal spinal cord",
abstract = "The Luxol fast blue (LFB) technique is widely used for the assessment of myelination. Lectin histochemistry using peanut agglutinin (PNA) has also been employed for this purpose. Recently, immunohistochemical methods using antibodies to several myelin-related proteins have been adopted to study myelination in humans. However, the relative sensitivities of these different methods for the detection of early myelination in the human fetal central nervous system have not been determined. Vibratome sections of cervical spinal cord from 15 human abortuses ranging in age from 15 to 24 gestational weeks (GW) were probed with immunohistochemical methods using antibodies to myelin basic protein (MBP), 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), and myelin-associated glycoprotein (MAG). In addition, LFB and PNA histochemistry was employed. The degree of myelination observed in immunohistochemically stained sections was compared to that found in corresponding LFB- and PNA-stained paraffin-embedded tissues. The intensity of myelination was graded by two observers on a scale of 0 (none), +1 (mild), +2 (moderate), and +3 (marked). At all ages examined, the MBP immunohistochemical method revealed more myelin than LFB or MAG staining. CNPase could not be reliably detected until after 18 GW. Peanut agglutinin stained myelin, but subpial astrocytes and the intervening neuropil were also stained. These results suggest that MBP is a more sensitive marker for early human fetal myelination than CNPase, MAG, PNA or LFB.",
keywords = "Central nervous system, Immunohistochemistry, Myelin proteins, Spinal cord",
author = "Bodhireddy, {Surender R.} and Lyman, {William D.} and Rashbaum, {William K.} and Weidenheim, {Karen M.}",
year = "1994",
language = "English (US)",
volume = "53",
pages = "144--149",
journal = "American Journal of Psychotherapy",
issn = "0002-9564",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Immunohistochemical detection of myelin basic protein is a sensitive marker of myelination in second trimester human fetal spinal cord

AU - Bodhireddy, Surender R.

AU - Lyman, William D.

AU - Rashbaum, William K.

AU - Weidenheim, Karen M.

PY - 1994

Y1 - 1994

N2 - The Luxol fast blue (LFB) technique is widely used for the assessment of myelination. Lectin histochemistry using peanut agglutinin (PNA) has also been employed for this purpose. Recently, immunohistochemical methods using antibodies to several myelin-related proteins have been adopted to study myelination in humans. However, the relative sensitivities of these different methods for the detection of early myelination in the human fetal central nervous system have not been determined. Vibratome sections of cervical spinal cord from 15 human abortuses ranging in age from 15 to 24 gestational weeks (GW) were probed with immunohistochemical methods using antibodies to myelin basic protein (MBP), 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), and myelin-associated glycoprotein (MAG). In addition, LFB and PNA histochemistry was employed. The degree of myelination observed in immunohistochemically stained sections was compared to that found in corresponding LFB- and PNA-stained paraffin-embedded tissues. The intensity of myelination was graded by two observers on a scale of 0 (none), +1 (mild), +2 (moderate), and +3 (marked). At all ages examined, the MBP immunohistochemical method revealed more myelin than LFB or MAG staining. CNPase could not be reliably detected until after 18 GW. Peanut agglutinin stained myelin, but subpial astrocytes and the intervening neuropil were also stained. These results suggest that MBP is a more sensitive marker for early human fetal myelination than CNPase, MAG, PNA or LFB.

AB - The Luxol fast blue (LFB) technique is widely used for the assessment of myelination. Lectin histochemistry using peanut agglutinin (PNA) has also been employed for this purpose. Recently, immunohistochemical methods using antibodies to several myelin-related proteins have been adopted to study myelination in humans. However, the relative sensitivities of these different methods for the detection of early myelination in the human fetal central nervous system have not been determined. Vibratome sections of cervical spinal cord from 15 human abortuses ranging in age from 15 to 24 gestational weeks (GW) were probed with immunohistochemical methods using antibodies to myelin basic protein (MBP), 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), and myelin-associated glycoprotein (MAG). In addition, LFB and PNA histochemistry was employed. The degree of myelination observed in immunohistochemically stained sections was compared to that found in corresponding LFB- and PNA-stained paraffin-embedded tissues. The intensity of myelination was graded by two observers on a scale of 0 (none), +1 (mild), +2 (moderate), and +3 (marked). At all ages examined, the MBP immunohistochemical method revealed more myelin than LFB or MAG staining. CNPase could not be reliably detected until after 18 GW. Peanut agglutinin stained myelin, but subpial astrocytes and the intervening neuropil were also stained. These results suggest that MBP is a more sensitive marker for early human fetal myelination than CNPase, MAG, PNA or LFB.

KW - Central nervous system

KW - Immunohistochemistry

KW - Myelin proteins

KW - Spinal cord

UR - http://www.scopus.com/inward/record.url?scp=0028206676&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028206676&partnerID=8YFLogxK

M3 - Article

C2 - 7509848

AN - SCOPUS:0028206676

VL - 53

SP - 144

EP - 149

JO - American Journal of Psychotherapy

JF - American Journal of Psychotherapy

SN - 0002-9564

IS - 2

ER -