Immunocytochemical localization of the neural-specific regulatory subunit of the type II cyclic AMP-dependent protein kinase to postsynaptic structures in the rat brain

Nandor Ludvig, Charles E. Ribak, John D. Scott, Charles S. Rubin

Research output: Contribution to journalArticle

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Abstract

The cellular and subcellular distribution of a major cyclic AMP binding protein in the central nervous system, the neural-specific regulatory subunit of the type II cyclic AMP-dependent protein kinase (RII-B), was analyzed in rat brains with light and electron microscopic immunocytochemical methods. The distribution of the non-neural isoform of the regulatory subunit of the enzyme (RII-H) was also analyzed. It was found that RII-B immunoreactivity was predominantly localized to neurons whereas glial and endothelial cells were unlabeled. In the neurons the RII-B immunoreactivity occurred in the perikaryal cytoplasm and in the dendrites; there was no significant accumulation of immunoreaction product in nuclei, myelinated axons and axon terminals. Although immunoreactivity was never detected in axon terminals, it was characteristically associated with the postsynaptic densities and the surrounding non-synaptic sites in somata, dendrites and dendritic spines. The localization of RII-B antigenic sites did not show specificity to any type of neuron or synapse, but the amount of immunoreactivity varied. The distribution of RII-H immunoreactivity was similar to that of RII-B except that RII-H immunoreaction product was also observed in glial cells and occurred more frequently in myelinated axons. Our data confirm that RII-B is one of the major cyclic AMP binding proteins in neurons, and provide morphological support for the involvement of the type II cyclic AMP-dependent protein kinase in postsynaptic neural functions.

Original languageEnglish (US)
Pages (from-to)90-102
Number of pages13
JournalBrain Research
Volume520
Issue number1-2
DOIs
StatePublished - Jun 18 1990

Fingerprint

Cyclic AMP-Dependent Protein Kinase Type II
Cyclic AMP
Neurons
Presynaptic Terminals
Brain
Dendrites
Neuroglia
Axons
Carrier Proteins
Post-Synaptic Density
Proto-Oncogene Proteins c-akt
Dendritic Spines
Carisoprodol
Synapses
Protein Isoforms
Cytoplasm
Central Nervous System
Endothelial Cells
Electrons
Light

Keywords

  • Cyclic adenosine monophosphate
  • Electron microscopy
  • Immunocytochemistry
  • Postsynaptic site
  • Protein kinase
  • Regulatory subunit

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Immunocytochemical localization of the neural-specific regulatory subunit of the type II cyclic AMP-dependent protein kinase to postsynaptic structures in the rat brain. / Ludvig, Nandor; Ribak, Charles E.; Scott, John D.; Rubin, Charles S.

In: Brain Research, Vol. 520, No. 1-2, 18.06.1990, p. 90-102.

Research output: Contribution to journalArticle

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