Immunochemical Detection of Guanine Nucleotide Binding Proteins Mono-ADP-ribosylated by Bacterial Toxins

B. Eide, P. Gierschik, A. Spiegel

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Rabbits immunized with ADP-ribose chemically conjugated to carrier proteins developed antibodies reactive against guanine nucleotide binding proteins (G proteins) that had been mono-ADP-ribosylated by bacterial toxins. Antibody reactivity on immunoblots was strictly dependent on incubation of substrate proteins with both toxin and NAD and was quantitatively related to the extent of ADP-ribosylation. Gi, G0, and transducin (ADP-ribosylated by pertussis toxin) and elongation factor II (EF-II) (ADP-ribosylated by pseudomonas exotoxin) all reacted with ADP-ribose antibodies. ADP-ribose antibodies detected the ADP-ribosylation of an approximately 40-kilodalton (kDa) membrane protein related to Gj in intact human neutrophils incubated with pertussis toxin and the ADP-ribosylation of an approximately 90-kDa cytosolic protein, presumably EF-II, in intact HUT-102 cells incubated with pseudomonas exotoxin. ADP-ribose antibodies represent a novel tool for the identification and study of G proteins and other substrates for bacterial toxin ADP-ribosylation.

Original languageEnglish (US)
Pages (from-to)6711-6715
Number of pages5
JournalBiochemistry
Volume25
Issue number21
DOIs
StatePublished - Oct 1986
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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