Immuno-Modulatory Effect and Therapeutic Potential of Brugia malayi Cystatin in Experimentally Induced Arthritis

Ravi Shankar Prasad Yadav; Vishal Khatri; Nitin Amdare; Kalyan Goswami; V. B. Shivkumar; Nitin Gangane; Maryada Venkata Rami Reddy

doi:10.1007/s12291-015-0515-z

Immuno-Modulatory Effect and Therapeutic Potential of Brugia malayi Cystatin in Experimentally Induced Arthritis

Ravi Shankar Prasad Yadav, Vishal Khatri, Nitin Amdare, Kalyan Goswami, V. B. Shivkumar, Nitin Gangane, Maryada Venkata Rami Reddy

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Helminths are known to modulate host’s immune system and understanding this modulation can help in identification of novel therapeutic agents for autoimmune diseases. In this study, we have assessed the immune-modulatory activity and the therapeutic effect of Brugia malayi recombinant cystatin (rBmCys) in methylated BSA (mBSA) induced arthritis using rodent model. Administration of rBmCys has suppressed the severity of mBSA-arthritis in mastomys by reducing paw swelling and other clinical disease parameters as evident from significantly decreased arthritic index. The anti-arthritic effect of rBmCys was also confirmed by decreased histopathological score for synovitis, bone erosion and fibrosis in the tissue sections of paws. Further, this therapeutic effect of cystatin was found to be associated with significantly decreased production of IFN-γ and TNF-α and increased release of IL-4 and IL-10 cytokines. These results implied that rBmCys treatment has alleviated mBSA-induced arthritis and thus can be a promising alternative agent for the treatment of arthritis.

Original language	English (US)
Pages (from-to)	203-208
Number of pages	6
Journal	Indian Journal of Clinical Biochemistry
Volume	31
Issue number	2
DOIs	https://doi.org/10.1007/s12291-015-0515-z
State	Published - Apr 1 2016
Externally published	Yes

Keywords

Brugia malayi cystatin
Methylated BSA
Rheumatoid arthritis
Therapeutic effect

ASJC Scopus subject areas

Clinical Biochemistry

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10.1007/s12291-015-0515-z

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title = "Immuno-Modulatory Effect and Therapeutic Potential of Brugia malayi Cystatin in Experimentally Induced Arthritis",

abstract = "Helminths are known to modulate host{\textquoteright}s immune system and understanding this modulation can help in identification of novel therapeutic agents for autoimmune diseases. In this study, we have assessed the immune-modulatory activity and the therapeutic effect of Brugia malayi recombinant cystatin (rBmCys) in methylated BSA (mBSA) induced arthritis using rodent model. Administration of rBmCys has suppressed the severity of mBSA-arthritis in mastomys by reducing paw swelling and other clinical disease parameters as evident from significantly decreased arthritic index. The anti-arthritic effect of rBmCys was also confirmed by decreased histopathological score for synovitis, bone erosion and fibrosis in the tissue sections of paws. Further, this therapeutic effect of cystatin was found to be associated with significantly decreased production of IFN-γ and TNF-α and increased release of IL-4 and IL-10 cytokines. These results implied that rBmCys treatment has alleviated mBSA-induced arthritis and thus can be a promising alternative agent for the treatment of arthritis.",

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author = "Yadav, {Ravi Shankar Prasad} and Vishal Khatri and Nitin Amdare and Kalyan Goswami and Shivkumar, {V. B.} and Nitin Gangane and Reddy, {Maryada Venkata Rami}",

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doi = "10.1007/s12291-015-0515-z",

language = "English (US)",

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AU - Yadav, Ravi Shankar Prasad

AU - Khatri, Vishal

AU - Amdare, Nitin

AU - Goswami, Kalyan

AU - Shivkumar, V. B.

AU - Gangane, Nitin

AU - Reddy, Maryada Venkata Rami

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Helminths are known to modulate host’s immune system and understanding this modulation can help in identification of novel therapeutic agents for autoimmune diseases. In this study, we have assessed the immune-modulatory activity and the therapeutic effect of Brugia malayi recombinant cystatin (rBmCys) in methylated BSA (mBSA) induced arthritis using rodent model. Administration of rBmCys has suppressed the severity of mBSA-arthritis in mastomys by reducing paw swelling and other clinical disease parameters as evident from significantly decreased arthritic index. The anti-arthritic effect of rBmCys was also confirmed by decreased histopathological score for synovitis, bone erosion and fibrosis in the tissue sections of paws. Further, this therapeutic effect of cystatin was found to be associated with significantly decreased production of IFN-γ and TNF-α and increased release of IL-4 and IL-10 cytokines. These results implied that rBmCys treatment has alleviated mBSA-induced arthritis and thus can be a promising alternative agent for the treatment of arthritis.

AB - Helminths are known to modulate host’s immune system and understanding this modulation can help in identification of novel therapeutic agents for autoimmune diseases. In this study, we have assessed the immune-modulatory activity and the therapeutic effect of Brugia malayi recombinant cystatin (rBmCys) in methylated BSA (mBSA) induced arthritis using rodent model. Administration of rBmCys has suppressed the severity of mBSA-arthritis in mastomys by reducing paw swelling and other clinical disease parameters as evident from significantly decreased arthritic index. The anti-arthritic effect of rBmCys was also confirmed by decreased histopathological score for synovitis, bone erosion and fibrosis in the tissue sections of paws. Further, this therapeutic effect of cystatin was found to be associated with significantly decreased production of IFN-γ and TNF-α and increased release of IL-4 and IL-10 cytokines. These results implied that rBmCys treatment has alleviated mBSA-induced arthritis and thus can be a promising alternative agent for the treatment of arthritis.

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KW - Therapeutic effect

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Immuno-Modulatory Effect and Therapeutic Potential of Brugia malayi Cystatin in Experimentally Induced Arthritis

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