Immune signatures of protective spleen memory CD8 T cells

Lilia Brinza, Sophia Djebali, Martine Tomkowiak, Julien Mafille, Céline Loiseau, Pierre Emmanuel Jouve, Simon De Bernard, Laurent Buffat, Bruno Lina, Michèle Ottmann, Manuel Rosa-Calatrava, Stéphane Schicklin, Nathalie Bonnefoy, Gregoire Lauvau, Morgan Grau, Mélanie Wencker, Christophe Arpin, Thierry Walzer, Yann Leverrier, Jacqueline Marvel

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Memory CD8 T lymphocyte populations are remarkably heterogeneous and differ in their ability to protect the host. In order to identify the whole range of qualities uniquely associated with protective memory cells we compared the gene expression signatures of two qualities of memory CD8 T cells sharing the same antigenic-specificity: protective (Influenza-induced, Flu-TM) and non-protective (peptide-induced, TIM) spleen memory CD8 T cells. Although Flu-TM and TIM express classical phenotypic memory markers and are polyfunctional, only Flu-TM protects against a lethal viral challenge. Protective memory CD8 T cells express a unique set of genes involved in migration and survival that correlate with their unique capacity to rapidly migrate within the infected lung parenchyma in response to influenza infection. We also enlighten a new set of poised genes expressed by protective cells that is strongly enriched in cytokines and chemokines such as Ccl1, Ccl9 and Gm-csf. CCL1 and GM-CSF genes are also poised in human memory CD8 T cells. These immune signatures are also induced by two other pathogens (vaccinia virus and Listeria monocytogenes). The immune signatures associated with immune protection were identified on circulating cells, i.e. those that are easily accessible for immuno-monitoring and could help predict vaccines efficacy.

Original languageEnglish (US)
Article number37651
JournalScientific Reports
Volume6
DOIs
StatePublished - Nov 24 2016

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Spleen
T-Lymphocytes
Human Influenza
Genes
Aptitude
Vaccinia virus
Listeria monocytogenes
Granulocyte-Macrophage Colony-Stimulating Factor
Transcriptome
Chemokines
Epitopes
Vaccines
Cytokines
Lung
Peptides
Survival
Infection
Population

ASJC Scopus subject areas

  • General

Cite this

Brinza, L., Djebali, S., Tomkowiak, M., Mafille, J., Loiseau, C., Jouve, P. E., ... Marvel, J. (2016). Immune signatures of protective spleen memory CD8 T cells. Scientific Reports, 6, [37651]. https://doi.org/10.1038/srep37651

Immune signatures of protective spleen memory CD8 T cells. / Brinza, Lilia; Djebali, Sophia; Tomkowiak, Martine; Mafille, Julien; Loiseau, Céline; Jouve, Pierre Emmanuel; De Bernard, Simon; Buffat, Laurent; Lina, Bruno; Ottmann, Michèle; Rosa-Calatrava, Manuel; Schicklin, Stéphane; Bonnefoy, Nathalie; Lauvau, Gregoire; Grau, Morgan; Wencker, Mélanie; Arpin, Christophe; Walzer, Thierry; Leverrier, Yann; Marvel, Jacqueline.

In: Scientific Reports, Vol. 6, 37651, 24.11.2016.

Research output: Contribution to journalArticle

Brinza, L, Djebali, S, Tomkowiak, M, Mafille, J, Loiseau, C, Jouve, PE, De Bernard, S, Buffat, L, Lina, B, Ottmann, M, Rosa-Calatrava, M, Schicklin, S, Bonnefoy, N, Lauvau, G, Grau, M, Wencker, M, Arpin, C, Walzer, T, Leverrier, Y & Marvel, J 2016, 'Immune signatures of protective spleen memory CD8 T cells', Scientific Reports, vol. 6, 37651. https://doi.org/10.1038/srep37651
Brinza L, Djebali S, Tomkowiak M, Mafille J, Loiseau C, Jouve PE et al. Immune signatures of protective spleen memory CD8 T cells. Scientific Reports. 2016 Nov 24;6. 37651. https://doi.org/10.1038/srep37651
Brinza, Lilia ; Djebali, Sophia ; Tomkowiak, Martine ; Mafille, Julien ; Loiseau, Céline ; Jouve, Pierre Emmanuel ; De Bernard, Simon ; Buffat, Laurent ; Lina, Bruno ; Ottmann, Michèle ; Rosa-Calatrava, Manuel ; Schicklin, Stéphane ; Bonnefoy, Nathalie ; Lauvau, Gregoire ; Grau, Morgan ; Wencker, Mélanie ; Arpin, Christophe ; Walzer, Thierry ; Leverrier, Yann ; Marvel, Jacqueline. / Immune signatures of protective spleen memory CD8 T cells. In: Scientific Reports. 2016 ; Vol. 6.
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