Immune Profiling of Plasma and Cervical Secretions Using Recycling Immunoaffinity Chromatography

Philip E. Castle, Terry M. Phillips, Allan Hildesheim, Rolando Herrero, M. Concepcion Bratti, Ana Cecilia Rodríguez, Lidia Ana Morera, Ruth Pfeiffer, Martha L. Hutchinson, Ligia A. Pinto, Mark Schiffman

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Small volumes of cervical secretions have limited measurements of immunity at the cervix, which may be important to studies of human papillomavirus (HPV)(RPV). We report the use of recycling immunoaffinity chromatography to efficiently study immune profiles in cervical secretions. Frozen pairs of plasma and cervical secretions (collected on ophthalmic sponges) were selected randomly from women with normal cervical cytology (n = 50) participating in a natural history study of HPV in Guanacaste, Costa Rica. Single 25-μ1 aliquots of plasma and (diluted) cervical secretions were assayed for interleukin (IL) -1β, -2, -4, -6, -8, -10, -12, -13, -15, IFN-α, -β, -γ, tumor necrosis factor-α, -β, RANTES (regulated on activation normal T-cell express and secreted), MCP-1 (monocyte chemoattractant protein), -2, -3, macrophage inflammatory protein-1α, -β (regulated on activation normal T-cell express and secreted), macrophage colony-stimulating factor, IgG, IgA, and cyclooxygenase 2. All of the specimens were tested as blind replicates, and refrozen plasma was retested 4 months later. To evaluate the reproducibility of the repeat measurements and to examine the correlation between plasma and cervical secretions, we calculated κ values with 95% confidence intervals among categorized analyte values and Spearman correlation coefficients (ρ) among detectable, continuous analyte values. Measurements of all of the analytes in either plasma or cervical secretions were highly reproducible, with all of the κ ≥ 0.78 (70% above 0.90), and all of the ρ ≥ 0.88 (96% above 0.90). Only IL-1β (κ = 0.60 and ρ = 0.82) and IL-6 (κ = 0.50 and ρ = 0.78) levels were strongly correlated between plasma and cervical secretions. IFN-γ, tumor necrosis factor-β, RANTES, MCP-1, MCP-2, macrophage inflammatory protein-1α, and macrophage colony-stimulating factor levels were especially poorly correlated between plasma and cervical secretions (κ ≤ 0.25 and ρ ≤ 0.25). We conclude that recycling immunoaffinity chromatography is a reproducible method of measuring immune profiles from biological specimens, and immune profiles are not well correlated between plasma and cervical secretions, perhaps necessitating cervical collections to study cervix-specific immunity in HPV natural history studies.

Original languageEnglish (US)
Pages (from-to)1449-1456
Number of pages8
JournalCancer Epidemiology Biomarkers and Prevention
Volume12
Issue number12
StatePublished - Dec 2003
Externally publishedYes

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Recycling
Chromatography
Macrophage Inflammatory Proteins
Macrophage Colony-Stimulating Factor
Natural History
T-Lymphocytes
Interleukin-1
Cervix Uteri
Chemokine CCL8
Immunity
Chemokine CCL7
Tumor Necrosis Factor-alpha
Costa Rica
Porifera
Cyclooxygenase 2
Immunoglobulin A
Interleukin-2
Cell Biology
Interleukin-6
Immunoglobulin G

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Castle, P. E., Phillips, T. M., Hildesheim, A., Herrero, R., Bratti, M. C., Rodríguez, A. C., ... Schiffman, M. (2003). Immune Profiling of Plasma and Cervical Secretions Using Recycling Immunoaffinity Chromatography. Cancer Epidemiology Biomarkers and Prevention, 12(12), 1449-1456.

Immune Profiling of Plasma and Cervical Secretions Using Recycling Immunoaffinity Chromatography. / Castle, Philip E.; Phillips, Terry M.; Hildesheim, Allan; Herrero, Rolando; Bratti, M. Concepcion; Rodríguez, Ana Cecilia; Morera, Lidia Ana; Pfeiffer, Ruth; Hutchinson, Martha L.; Pinto, Ligia A.; Schiffman, Mark.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 12, No. 12, 12.2003, p. 1449-1456.

Research output: Contribution to journalArticle

Castle, PE, Phillips, TM, Hildesheim, A, Herrero, R, Bratti, MC, Rodríguez, AC, Morera, LA, Pfeiffer, R, Hutchinson, ML, Pinto, LA & Schiffman, M 2003, 'Immune Profiling of Plasma and Cervical Secretions Using Recycling Immunoaffinity Chromatography', Cancer Epidemiology Biomarkers and Prevention, vol. 12, no. 12, pp. 1449-1456.
Castle PE, Phillips TM, Hildesheim A, Herrero R, Bratti MC, Rodríguez AC et al. Immune Profiling of Plasma and Cervical Secretions Using Recycling Immunoaffinity Chromatography. Cancer Epidemiology Biomarkers and Prevention. 2003 Dec;12(12):1449-1456.
Castle, Philip E. ; Phillips, Terry M. ; Hildesheim, Allan ; Herrero, Rolando ; Bratti, M. Concepcion ; Rodríguez, Ana Cecilia ; Morera, Lidia Ana ; Pfeiffer, Ruth ; Hutchinson, Martha L. ; Pinto, Ligia A. ; Schiffman, Mark. / Immune Profiling of Plasma and Cervical Secretions Using Recycling Immunoaffinity Chromatography. In: Cancer Epidemiology Biomarkers and Prevention. 2003 ; Vol. 12, No. 12. pp. 1449-1456.
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