Imaging tumor cell movement in vivo

David Entenberg, Dmitriy Kedrin, Jeffrey Wyckoff, Erik Sahai, John Condeelis, Jeffrey E. Segall

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

This unit describes the methods that we have been developing for analyzing tumor cell motility in mouse and rat models of breast cancer metastasis. Rodents are commonly used both to provide a mammalian system for studying human tumor cells (as xenografts in immunocompromised mice) as well as for following the development of tumors from a specific tissue type in transgenic lines. The Basic Protocol in this unit describes the standard methods used for generation of mammary tumors and imaging them. Additional protocols for labeling macrophages, blood vessel imaging, and image analysis are also included.

Original languageEnglish (US)
Article number19.7
JournalCurrent Protocols in Cell Biology
Issue numberSUPPL.58
DOIs
StatePublished - Sep 4 2013

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Keywords

  • Image analysis
  • Imaging
  • Mammary tumor
  • Metastasis

ASJC Scopus subject areas

  • Cell Biology

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