Imaging tumor cell movement in vivo

David R. Entenberg, Dmitriy Kedrin, Jeffrey Wyckoff, Erik Sahai, John S. Condeelis, Jeffrey E. Segall

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

This unit describes the methods that we have been developing for analyzing tumor cell motility in mouse and rat models of breast cancer metastasis. Rodents are commonly used both to provide a mammalian system for studying human tumor cells (as xenografts in immunocompromised mice) as well as for following the development of tumors from a specific tissue type in transgenic lines. The Basic Protocol in this unit describes the standard methods used for generation of mammary tumors and imaging them. Additional protocols for labeling macrophages, blood vessel imaging, and image analysis are also included.

Original languageEnglish (US)
Article number19.7
JournalCurrent Protocols in Cell Biology
Issue numberSUPPL.58
DOIs
StatePublished - 2013

Fingerprint

Cell Movement
Breast Neoplasms
Neoplasms
Heterografts
Blood Vessels
Rodentia
Macrophages
Neoplasm Metastasis

Keywords

  • Image analysis
  • Imaging
  • Mammary tumor
  • Metastasis

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Cite this

Imaging tumor cell movement in vivo. / Entenberg, David R.; Kedrin, Dmitriy; Wyckoff, Jeffrey; Sahai, Erik; Condeelis, John S.; Segall, Jeffrey E.

In: Current Protocols in Cell Biology, No. SUPPL.58, 19.7, 2013.

Research output: Contribution to journalArticle

@article{18110d292b504a538533c694674d1051,
title = "Imaging tumor cell movement in vivo",
abstract = "This unit describes the methods that we have been developing for analyzing tumor cell motility in mouse and rat models of breast cancer metastasis. Rodents are commonly used both to provide a mammalian system for studying human tumor cells (as xenografts in immunocompromised mice) as well as for following the development of tumors from a specific tissue type in transgenic lines. The Basic Protocol in this unit describes the standard methods used for generation of mammary tumors and imaging them. Additional protocols for labeling macrophages, blood vessel imaging, and image analysis are also included.",
keywords = "Image analysis, Imaging, Mammary tumor, Metastasis",
author = "Entenberg, {David R.} and Dmitriy Kedrin and Jeffrey Wyckoff and Erik Sahai and Condeelis, {John S.} and Segall, {Jeffrey E.}",
year = "2013",
doi = "10.1002/0471143030.cb1907s58",
language = "English (US)",
journal = "Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]",
issn = "1934-2500",
publisher = "John Wiley and Sons Inc.",
number = "SUPPL.58",

}

TY - JOUR

T1 - Imaging tumor cell movement in vivo

AU - Entenberg, David R.

AU - Kedrin, Dmitriy

AU - Wyckoff, Jeffrey

AU - Sahai, Erik

AU - Condeelis, John S.

AU - Segall, Jeffrey E.

PY - 2013

Y1 - 2013

N2 - This unit describes the methods that we have been developing for analyzing tumor cell motility in mouse and rat models of breast cancer metastasis. Rodents are commonly used both to provide a mammalian system for studying human tumor cells (as xenografts in immunocompromised mice) as well as for following the development of tumors from a specific tissue type in transgenic lines. The Basic Protocol in this unit describes the standard methods used for generation of mammary tumors and imaging them. Additional protocols for labeling macrophages, blood vessel imaging, and image analysis are also included.

AB - This unit describes the methods that we have been developing for analyzing tumor cell motility in mouse and rat models of breast cancer metastasis. Rodents are commonly used both to provide a mammalian system for studying human tumor cells (as xenografts in immunocompromised mice) as well as for following the development of tumors from a specific tissue type in transgenic lines. The Basic Protocol in this unit describes the standard methods used for generation of mammary tumors and imaging them. Additional protocols for labeling macrophages, blood vessel imaging, and image analysis are also included.

KW - Image analysis

KW - Imaging

KW - Mammary tumor

KW - Metastasis

UR - http://www.scopus.com/inward/record.url?scp=84881171594&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881171594&partnerID=8YFLogxK

U2 - 10.1002/0471143030.cb1907s58

DO - 10.1002/0471143030.cb1907s58

M3 - Article

C2 - 23456602

AN - SCOPUS:84881171594

JO - Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]

JF - Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]

SN - 1934-2500

IS - SUPPL.58

M1 - 19.7

ER -