Imaging translation in single cells using fluorescent microscopy

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The regulation of translation provides a mechanism to control not only the abundance of proteins, but also the precise time and subcellular location that they are synthesized. Much of what is known concerning the molecular basis for translational control has been gleaned from experiments (e.g., luciferase assays and polysome analysis) that measure average changes in the protein synthesis of a population of cells, however, mechanistic insights can be obscured in ensemble measurements. The development of fluorescent microscopy techniques and reagents has allowed translation to be studied within its cellular context. Here we highlight recent methodologies that can be used to study global changes in protein synthesis or regulation of specific mRNAs in single cells. Imaging of translation has provided direct evidence for local translation of mRNAs at synapses in neurons and will become an important tool for studying translational control.

Original languageEnglish (US)
JournalCold Spring Harbor perspectives in biology
Volume4
Issue number11
DOIs
StatePublished - Nov 2012

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Microscopy
Microscopic examination
Imaging techniques
Messenger RNA
Proteins
Polyribosomes
Protein Biosynthesis
Luciferases
Synapses
Neurons
Assays
Cells
Population
Experiments

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Imaging translation in single cells using fluorescent microscopy. / Chao, Jeffrey A.; Yoon, Young J.; Singer, Robert H.

In: Cold Spring Harbor perspectives in biology, Vol. 4, No. 11, 11.2012.

Research output: Contribution to journalArticle

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