TY - GEN
T1 - Imaging transcription
T2 - 21st Century Genetics Genes at Work, 2015
AU - Coleman, Robert A.
AU - Liu, Zhe
AU - Darzacq, Xavier
AU - Tjian, Robert
AU - Singer, obert H.
AU - Lionnet, Timothée
N1 - Publisher Copyright:
© 2015 Cold Spring Harbor Laboratory Press.
PY - 2016
Y1 - 2016
N2 - Transcription, the first step of gene expression, is exquisitely regulated in higher eukaryotes to ensure correct development and homeostasis. Traditional biochemical, genetic, and genomic approaches have proved successful at identifying factors, regulatory sequences, and potential pathways that modulate transcription. However, they typically only provide snapshots or population averages of the highly dynamic, stochastic biochemical processes involved in transcriptional regulation. Singlemolecule live-cell imaging has, therefore, emerged as a complementary approach capable of circumventing these limitations. By observing sequences of molecular events in real time as they occur in their native context, imaging has the power to derive cause-and-effect relationships and quantitative kinetics to build predictive models of transcription. Ongoing progress in fluorescence imaging technology has brought new microscopes and labeling technologies that now make it possible to visualize and quantify the transcription process with single-molecule resolution in living cells and animals. Here we provide an overview of the evolution and current state of transcription imaging technologies. We discuss some of the important concepts they uncovered and present possible future developments that might solve long-standing questions in transcriptional regulation.
AB - Transcription, the first step of gene expression, is exquisitely regulated in higher eukaryotes to ensure correct development and homeostasis. Traditional biochemical, genetic, and genomic approaches have proved successful at identifying factors, regulatory sequences, and potential pathways that modulate transcription. However, they typically only provide snapshots or population averages of the highly dynamic, stochastic biochemical processes involved in transcriptional regulation. Singlemolecule live-cell imaging has, therefore, emerged as a complementary approach capable of circumventing these limitations. By observing sequences of molecular events in real time as they occur in their native context, imaging has the power to derive cause-and-effect relationships and quantitative kinetics to build predictive models of transcription. Ongoing progress in fluorescence imaging technology has brought new microscopes and labeling technologies that now make it possible to visualize and quantify the transcription process with single-molecule resolution in living cells and animals. Here we provide an overview of the evolution and current state of transcription imaging technologies. We discuss some of the important concepts they uncovered and present possible future developments that might solve long-standing questions in transcriptional regulation.
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U2 - 10.1101/sqb.2015.80.027201
DO - 10.1101/sqb.2015.80.027201
M3 - Conference contribution
C2 - 26763984
AN - SCOPUS:84978686269
SN - 9781621821472
T3 - Cold Spring Harbor Symposia on Quantitative Biology
SP - 1
EP - 8
BT - 21st Century Genetics Genes at Work, 2015
A2 - Grodzicker, Terri
A2 - Stillman, Bruce
A2 - Stewart, David
PB - Cold Spring Harbor Laboratory Press
Y2 - 26 May 2015 through 31 May 2015
ER -