Imaging transcription in living cells

Xavier Darzacq, Jie Yao, Daniel R. Larson, Sébastien Z. Causse, Lana Bosanac, Valeria De Turris, Vera M. Ruda, Timothee Lionnet, Daniel Zenklusen, Benjamin Guglielmi, Robert Tjian, Robert H. Singer

Research output: Contribution to journalReview article

94 Scopus citations

Abstract

The advent of new technologies for the imaging of living cells has made it possible to determine the properties of transcription, the kinetics of polymerase movement, the association of transcription factors, and the progression of the polymerase on the gene.We report here the current state of the field and the progress necessary to achieve a more complete understanding of the various steps in transcription. Our Consortium is dedicated to developing and implementing the technology to further this understanding.

Original languageEnglish (US)
Pages (from-to)173-196
Number of pages24
JournalAnnual Review of Biophysics
Volume38
Issue number1
DOIs
StatePublished - Nov 26 2009

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Keywords

  • Modeling
  • Polymerase kinetics
  • RNA synthesis in live cells

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Bioengineering
  • Biochemistry
  • Cell Biology

Cite this

Darzacq, X., Yao, J., Larson, D. R., Causse, S. Z., Bosanac, L., De Turris, V., Ruda, V. M., Lionnet, T., Zenklusen, D., Guglielmi, B., Tjian, R., & Singer, R. H. (2009). Imaging transcription in living cells. Annual Review of Biophysics, 38(1), 173-196. https://doi.org/10.1146/annurev.biophys.050708.133728