Imaging transcription in living cells

Xavier Darzacq; Jie Yao; Daniel R. Larson; Sébastien Z. Causse; Lana Bosanac; Valeria De Turris; Vera M. Ruda; Timothee Lionnet; Daniel Zenklusen; Benjamin Guglielmi; Robert Tjian; Robert H. Singer

doi:10.1146/annurev.biophys.050708.133728

Imaging transcription in living cells

Xavier Darzacq, Jie Yao, Daniel R. Larson, Sébastien Z. Causse, Lana Bosanac, Valeria De Turris, Vera M. Ruda, Timothee Lionnet, Daniel Zenklusen, Benjamin Guglielmi, Robert Tjian, Robert H. Singer

Cell Biology

Research output: Contribution to journal › Review article › peer-review

102 Scopus citations

Abstract

The advent of new technologies for the imaging of living cells has made it possible to determine the properties of transcription, the kinetics of polymerase movement, the association of transcription factors, and the progression of the polymerase on the gene.We report here the current state of the field and the progress necessary to achieve a more complete understanding of the various steps in transcription. Our Consortium is dedicated to developing and implementing the technology to further this understanding.

Original language	English (US)
Pages (from-to)	173-196
Number of pages	24
Journal	Annual Review of Biophysics
Volume	38
Issue number	1
DOIs	https://doi.org/10.1146/annurev.biophys.050708.133728
State	Published - 2009

Keywords

Modeling
Polymerase kinetics
RNA synthesis in live cells

ASJC Scopus subject areas

Biophysics
Structural Biology
Bioengineering
Biochemistry
Cell Biology

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10.1146/annurev.biophys.050708.133728

Cite this

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title = "Imaging transcription in living cells",

abstract = "The advent of new technologies for the imaging of living cells has made it possible to determine the properties of transcription, the kinetics of polymerase movement, the association of transcription factors, and the progression of the polymerase on the gene.We report here the current state of the field and the progress necessary to achieve a more complete understanding of the various steps in transcription. Our Consortium is dedicated to developing and implementing the technology to further this understanding.",

keywords = "Modeling, Polymerase kinetics, RNA synthesis in live cells",

author = "Xavier Darzacq and Jie Yao and Larson, {Daniel R.} and Causse, {S{\'e}bastien Z.} and Lana Bosanac and {De Turris}, Valeria and Ruda, {Vera M.} and Timothee Lionnet and Daniel Zenklusen and Benjamin Guglielmi and Robert Tjian and Singer, {Robert H.}",

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language = "English (US)",

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pages = "173--196",

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TY - JOUR

T1 - Imaging transcription in living cells

AU - Darzacq, Xavier

AU - Yao, Jie

AU - Larson, Daniel R.

AU - Causse, Sébastien Z.

AU - Bosanac, Lana

AU - De Turris, Valeria

AU - Ruda, Vera M.

AU - Lionnet, Timothee

AU - Zenklusen, Daniel

AU - Guglielmi, Benjamin

AU - Tjian, Robert

AU - Singer, Robert H.

PY - 2009

Y1 - 2009

N2 - The advent of new technologies for the imaging of living cells has made it possible to determine the properties of transcription, the kinetics of polymerase movement, the association of transcription factors, and the progression of the polymerase on the gene.We report here the current state of the field and the progress necessary to achieve a more complete understanding of the various steps in transcription. Our Consortium is dedicated to developing and implementing the technology to further this understanding.

AB - The advent of new technologies for the imaging of living cells has made it possible to determine the properties of transcription, the kinetics of polymerase movement, the association of transcription factors, and the progression of the polymerase on the gene.We report here the current state of the field and the progress necessary to achieve a more complete understanding of the various steps in transcription. Our Consortium is dedicated to developing and implementing the technology to further this understanding.

KW - Modeling

KW - Polymerase kinetics

KW - RNA synthesis in live cells

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DO - 10.1146/annurev.biophys.050708.133728

M3 - Review article

C2 - 19416065

AN - SCOPUS:67650328126

SN - 1936-122X

VL - 38

SP - 173

EP - 196

JO - Annual Review of Biophysics

JF - Annual Review of Biophysics

IS - 1

ER -

Imaging transcription in living cells

Abstract

Keywords

ASJC Scopus subject areas

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