Imaging Single mRNA Molecules in Mammalian Cells Using an Optimized MS2-MCP System

Maria Vera, Evelina Tutucci, Robert H. Singer

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Visualization of single mRNAs in their native cellular environment provides key information to study gene expression regulation. This fundamental biological question triggered the development of the MS2-MCP (MS2-Capsid Protein) system to tag mRNAs and image their life cycle using widefield fluorescence microscopy. The last two decades have evolved toward improving the qualitative and quantitative characteristics of the MS2-MCP system. Here, we provide a protocol to use the latest versions, MS2V6 and MS2V7, to tag and visualize mRNAs in mammalian cells in culture. The motivation behind engineering MS2V6 and MS2V7 was to overcome a degradation caveat observed in S. cerevisiae with the previous MS2-MCP systems. While for yeast we recommend the use of MS2V6, we found that for live-cell imaging experiments in mammalian cells, the MS2V7 has improved reporter properties.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages3-20
Number of pages18
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume2038
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • MS2 system
  • MS2V6
  • MS2V7
  • Quantitative fluorescence microscopy
  • Short-lived mRNAs
  • Single cell
  • Single-molecule imaging
  • smFISH

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Vera, M., Tutucci, E., & Singer, R. H. (2019). Imaging Single mRNA Molecules in Mammalian Cells Using an Optimized MS2-MCP System. In Methods in Molecular Biology (pp. 3-20). (Methods in Molecular Biology; Vol. 2038). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9674-2_1