Imaging Organization of RNA Processing within the Nucleus

Jeetayu Biswas; Weihan Li; Robert H. Singer; Robert A. Coleman

doi:10.1101/cshperspect.a039453

Imaging Organization of RNA Processing within the Nucleus

Jeetayu Biswas, Weihan Li, Robert H. Singer, Robert A. Coleman

Anatomy & Structural Biology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Within the nucleus, messenger RNA is generated and processed in a highly organized and regulated manner. Messenger RNA processing begins during transcription initiation and continues until the RNA is translated and degraded. Processes such as 5⁰ capping, alternative splicing, and 3⁰ end processing have been studied extensively with biochemical methods and more recently with single-molecule imaging approaches. In this review, we highlight how imaging has helped understand the highly dynamic process of RNA processing. We conclude with open questions and new technological developments that may further our understanding of RNA processing.

Original language	English (US)
Article number	a039453
Journal	Cold Spring Harbor Perspectives in Biology
Volume	13
Issue number	12
DOIs	https://doi.org/10.1101/cshperspect.a039453
State	Published - Dec 2021

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1101/cshperspect.a039453

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title = "Imaging Organization of RNA Processing within the Nucleus",

abstract = "Within the nucleus, messenger RNA is generated and processed in a highly organized and regulated manner. Messenger RNA processing begins during transcription initiation and continues until the RNA is translated and degraded. Processes such as 50 capping, alternative splicing, and 30 end processing have been studied extensively with biochemical methods and more recently with single-molecule imaging approaches. In this review, we highlight how imaging has helped understand the highly dynamic process of RNA processing. We conclude with open questions and new technological developments that may further our understanding of RNA processing.",

author = "Jeetayu Biswas and Weihan Li and Singer, {Robert H.} and Coleman, {Robert A.}",

note = "Funding Information: The authors thank Justin C. Wheat and other members of the Einstein community as well as members of the Singer and Coleman laboratories, past and present, for their helpful discussions and comments. J.B. was supported with funding from an MSTP Training Grant T32GM007288 and predoctoral fellowship F30CA214009. W.L. and R.H.S. were supported by NIH Grants R01NS083085 and R35G M136296. R.A.C. was supported by NIH Grant R01GM126045. The authors acknowledge the members of the 4DN community and NIH U01DA047729. Publisher Copyright: {\textcopyright} 2021 Cold Spring Harbor Laboratory Press;.",

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AU - Singer, Robert H.

AU - Coleman, Robert A.

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Imaging Organization of RNA Processing within the Nucleus

Abstract

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