Imaging of transparent spheres through a planar interface using a high-numerical-aperture optical microscope

Ben Ovryn, Steven H. Izen

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The details of a model used to predict the scattering of a plane polarized wave by a spherical particle as observed with a microscope are presented. The model accounts for the effect of a refractive interface on the outgoing scattered field and determines the image produced by a lens with high numerical aperture. The predictions of the model are verified by direct comparison with the experimentally observed scattering from polystyrene spheres in a fluid.

Original languageEnglish (US)
Pages (from-to)1202-1214
Number of pages13
JournalJournal of the Optical Society of America A: Optics and Image Science, and Vision
Volume17
Issue number7
StatePublished - 2000
Externally publishedYes

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Microscopes
Imaging techniques
Scattering
Polystyrenes
Lenses
Fluids

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Computer Vision and Pattern Recognition

Cite this

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abstract = "The details of a model used to predict the scattering of a plane polarized wave by a spherical particle as observed with a microscope are presented. The model accounts for the effect of a refractive interface on the outgoing scattered field and determines the image produced by a lens with high numerical aperture. The predictions of the model are verified by direct comparison with the experimentally observed scattering from polystyrene spheres in a fluid.",
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