Imaging of transparent spheres through a planar interface using a high-numerical-aperture optical microscope

Ben Ovryn, Steven H. Izen

Research output: Contribution to journalArticle

52 Scopus citations


The details of a model used to predict the scattering of a plane polarized wave by a spherical particle as observed with a microscope are presented. The model accounts for the effect of a refractive interface on the outgoing scattered field and determines the image produced by a lens with high numerical aperture. The predictions of the model are verified by direct comparison with the experimentally observed scattering from polystyrene spheres in a fluid.

Original languageEnglish (US)
Pages (from-to)1202-1213
Number of pages12
JournalJournal of the Optical Society of America A: Optics and Image Science, and Vision
Issue number7
StatePublished - Jul 2000


ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Computer Vision and Pattern Recognition

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