Imaging of gene expression in living cells and tissues

Robert H. Singer, David S. Lawrence, Ben Ovryn, John Condeelis

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

It is possible to observe gene expression within single cells using a tetracycline inducible promoter for activation. Transcription can be observed by using a fluorescent fusion protein to bind nascent RNA. Ultimately, it is desirable to activate a reporter gene within a single cell with only photons. This is achieved by preparing a chemically altered transcription factor that is functionally unable to activate a reporter gene until it is exposed to photon excitation. We apply two-photon imaging to visualize tumor cells expressing a transgene and ultimately this approach will provide the means to activate a specific gene within a single cell within any tissue to ultimately observe its functional significance in situ.

Original languageEnglish (US)
Article number051406
JournalJournal of Biomedical Optics
Volume10
Issue number5
DOIs
StatePublished - Sep 1 2005

Keywords

  • Caged transcription factors
  • Gene expression
  • Noninvasive imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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