Imaging of gene expression in living cells and tissues

Robert H. Singer, David S. Lawrence, Ben Ovryn, John S. Condeelis

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

It is possible to observe gene expression within single cells using a tetracycline inducible promoter for activation. Transcription can be observed by using a fluorescent fusion protein to bind nascent RNA. Ultimately, it is desirable to activate a reporter gene within a single cell with only photons. This is achieved by preparing a chemically altered transcription factor that is functionally unable to activate a reporter gene until it is exposed to photon excitation. We apply two-photon imaging to visualize tumor cells expressing a transgene and ultimately this approach will provide the means to activate a specific gene within a single cell within any tissue to ultimately observe its functional significance in situ.

Original languageEnglish (US)
Article number051406
JournalJournal of Biomedical Optics
Volume10
Issue number5
DOIs
StatePublished - Sep 2005

Fingerprint

gene expression
Gene expression
genes
Photons
Genes
Cells
Tissue
Gene Expression
Imaging techniques
photons
cells
Reporter Genes
tetracyclines
Transcription factors
Transcription
Tetracycline
RNA
Tumors
Transcription Factors
Fusion reactions

Keywords

  • Caged transcription factors
  • Gene expression
  • Noninvasive imaging

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

Cite this

Imaging of gene expression in living cells and tissues. / Singer, Robert H.; Lawrence, David S.; Ovryn, Ben; Condeelis, John S.

In: Journal of Biomedical Optics, Vol. 10, No. 5, 051406, 09.2005.

Research output: Contribution to journalArticle

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