Imaging of DNA and RNA in Living Eukaryotic Cells to Reveal Spatiotemporal Dynamics of Gene Expression

Hanae Sato, Sulagna Das, Robert H. Singer, Robert H. Singer, Maria Vera, Maria Vera Ugalde

Research output: Contribution to journalReview articlepeer-review

11 Scopus citations

Abstract

This review focuses on imaging DNA and single RNA molecules in living cells to define eukaryotic functional organization and dynamic processes. The latest advances in technologies to visualize individual DNA loci and RNAs in real time are discussed. Single-molecule fluorescence microscopy provides the spatial and temporal resolution to reveal mechanisms regulating fundamental cell functions. Novel insights into the regulation of nuclear architecture, transcription, posttranscriptional RNA processing, and RNA localization provided by multicolor fluorescence microscopy are reviewed. A perspective on the future use of live imaging technologies and overcoming their current limitations is provided.

Original languageEnglish (US)
Pages (from-to)159-187
Number of pages29
JournalAnnual review of biochemistry
Volume89
DOIs
StatePublished - Jun 20 2020

Keywords

  • Fluorescence microscopy
  • nuclear organization
  • posttranscriptional regulation
  • single-molecule techniques
  • transcription

ASJC Scopus subject areas

  • Biochemistry

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