Imaging mRNA movement from transcription sites to translation sites

Alexis J. Rodriguez, John Condeelis, Robert H. Singer, Jason B. Dictenberg

Research output: Contribution to journalReview article

45 Scopus citations

Abstract

RNA localization is one mechanism to temporally and spatially restrict protein synthesis to specific subcellular compartments in response to extracellular stimuli. To understand the mechanisms of mRNA localization, a number of methods have been developed to follow the path of these molecules in living cells including direct labeling of target mRNAs, the MS2-GFP system, and molecular beacons. We review advances in these methods with the goal of identifying the particular strengths and weaknesses of the various approaches in their ability to follow the movements of mRNAs from transcription sites to translation sites.

Original languageEnglish (US)
Pages (from-to)202-208
Number of pages7
JournalSeminars in Cell and Developmental Biology
Volume18
Issue number2
DOIs
StatePublished - Apr 2007

Keywords

  • Fluorescent mRNAs
  • Live cell imaging
  • MS2
  • Molecular beacons

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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