Imaging mRNA in Vivo, from Birth to Death

Evelina Tutucci, Nathan M. Livingston, Robert H. Singer, Bin Wu

Research output: Contribution to journalReview articlepeer-review

42 Scopus citations

Abstract

RNA is the fundamental information transfer system in the cell. The ability to follow single messenger RNAs (mRNAs) from transcription to degradation with fluorescent probes gives quantitative information about how the information is transferred from DNA to proteins. This review focuses on the latest technological developments in the field of single-mRNA detection and their usage to study gene expression in both fixed and live cells. By describing the application of these imaging tools, we follow the journey of mRNA from transcription to decay in single cells, with single-molecule resolution. We review current theoretical models for describing transcription and translation that were generated by single-molecule and single-cell studies. These methods provide a basis to study how single-molecule interactions generate phenotypes, fundamentally changing our understating of gene expression regulation.

Original languageEnglish (US)
Pages (from-to)85-106
Number of pages22
JournalAnnual Review of Biophysics
Volume47
DOIs
StatePublished - May 20 2018

Keywords

  • gene expression
  • imaging
  • kinetics
  • single-cell
  • single-mRNA imaging
  • single-molecule

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Bioengineering
  • Biochemistry
  • Cell Biology

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