Imaging metals in: Caenorhabditis elegans

Michael Aschner, C. Palinski, M. Sperling, U. Karst, T. Schwerdtle, J. Bornhorst

Research output: Contribution to journalReview article

2 Citations (Scopus)

Abstract

Systemic trafficking and storage of essential metal ions play fundamental roles in living organisms by serving as essential cofactors in various cellular processes. Thereby metal quantification and localization are critical steps in understanding metal homeostasis, and how their dyshomeostasis might contribute to disease etiology and the ensuing pathologies. Furthermore, the amount and distribution of metals in organisms can provide insight into their underlying mechanisms of toxicity and toxicokinetics. While in vivo studies on metal imaging in mammalian experimental animals are complex, time- and resource-consuming, the nematode Caenorhabditis elegans (C. Elegans) provides a suitable comparative and complementary model system. Expressing homologous genes to those inherent to mammals, including those that regulate metal homeostasis and transport, C. Elegans has become a powerful tool to study metal homeostasis and toxicity. A number of recent technical advances have been made in the development and application of analytical methods to visualize metal ions in C. Elegans. Here, we briefly summarize key findings and challenges of the three main techniques and their application to the nematode, namely sensing fluorophores, microbeam synchrotron radiation X-ray fluorescence as well as laser ablation (LA) coupled to inductively coupled plasma-mass spectrometry (ICP-MS).

Original languageEnglish (US)
Pages (from-to)357-364
Number of pages8
JournalMetallomics
Volume9
Issue number4
DOIs
StatePublished - Apr 1 2017

Fingerprint

Caenorhabditis elegans
Metals
Imaging techniques
Homeostasis
Metal ions
Toxicity
Inductively coupled plasma mass spectrometry
Mammals
Fluorophores
Pathology
Laser ablation
Ions
Synchrotron radiation
Synchrotrons
Animals
Laser Therapy
Genes
Fluorescence
X rays
Mass Spectrometry

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Biophysics
  • Biochemistry
  • Biomaterials
  • Metals and Alloys

Cite this

Aschner, M., Palinski, C., Sperling, M., Karst, U., Schwerdtle, T., & Bornhorst, J. (2017). Imaging metals in: Caenorhabditis elegans. Metallomics, 9(4), 357-364. https://doi.org/10.1039/c6mt00265j

Imaging metals in : Caenorhabditis elegans. / Aschner, Michael; Palinski, C.; Sperling, M.; Karst, U.; Schwerdtle, T.; Bornhorst, J.

In: Metallomics, Vol. 9, No. 4, 01.04.2017, p. 357-364.

Research output: Contribution to journalReview article

Aschner, M, Palinski, C, Sperling, M, Karst, U, Schwerdtle, T & Bornhorst, J 2017, 'Imaging metals in: Caenorhabditis elegans', Metallomics, vol. 9, no. 4, pp. 357-364. https://doi.org/10.1039/c6mt00265j
Aschner M, Palinski C, Sperling M, Karst U, Schwerdtle T, Bornhorst J. Imaging metals in: Caenorhabditis elegans. Metallomics. 2017 Apr 1;9(4):357-364. https://doi.org/10.1039/c6mt00265j
Aschner, Michael ; Palinski, C. ; Sperling, M. ; Karst, U. ; Schwerdtle, T. ; Bornhorst, J. / Imaging metals in : Caenorhabditis elegans. In: Metallomics. 2017 ; Vol. 9, No. 4. pp. 357-364.
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