IL-22-producing CD4+ cells are depleted in actively inflamed colitis tissue

J. M. Leung, M. Davenport, M. J. Wolff, K. E. Wiens, W. M. Abidi, M. A. Poles, I. Cho, Thomas A. Ullman, L. Mayer, P. Loke

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

T helper type (Th17) cytokines such as interleukin (IL)-17A and IL-22 are important in maintaining mucosal barrier function and may be important in the pathogenesis of inflammatory bowel diseases (IBDs). Here, we analyzed cells from the colon of IBD patients and show that Crohn's disease (CD) patients had significantly elevated numbers of IL-17+, CD4+ cells compared with healthy controls and ulcerative colitis (UC) patients, but these numbers did not vary based on the inflammatory status of the mucosa. By contrast, UC patients had significantly reduced numbers of IL-22+ cells in actively inflamed tissues compared with both normal tissue and healthy controls. There was a selective increase in mono-IL-17-producing cells from the mucosa of UC patients with active inflammation together with increased expression of transforming growth factor (TGF)-β and c-Maf. Increasing concentrations of TGF-β in lamina propria mononuclear cell cultures significantly depleted Th22 cells, whereas anti-TGF-β antibodies increased IL-22 production. When mucosal microbiota was examined, depletion of Th22 cells in actively inflamed tissue was associated with reduced populations of Clostridiales and increased populations of Proteobacteria. These results suggest that increased TGF-β during active inflammation in UC may lead to the loss of Th22 cells in the human intestinal mucosa.

Original languageEnglish (US)
Pages (from-to)124-133
Number of pages10
JournalMucosal Immunology
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

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Colitis
Transforming Growth Factors
Ulcerative Colitis
Interleukin-17
Mucous Membrane
Inflammatory Bowel Diseases
Inflammation
Proteobacteria
Microbiota
Intestinal Mucosa
interleukin-22
Crohn Disease
Population
Colon
Cell Culture Techniques
Cytokines
Antibodies

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Leung, J. M., Davenport, M., Wolff, M. J., Wiens, K. E., Abidi, W. M., Poles, M. A., ... Loke, P. (2014). IL-22-producing CD4+ cells are depleted in actively inflamed colitis tissue. Mucosal Immunology, 7(1), 124-133. https://doi.org/10.1038/mi.2013.31

IL-22-producing CD4+ cells are depleted in actively inflamed colitis tissue. / Leung, J. M.; Davenport, M.; Wolff, M. J.; Wiens, K. E.; Abidi, W. M.; Poles, M. A.; Cho, I.; Ullman, Thomas A.; Mayer, L.; Loke, P.

In: Mucosal Immunology, Vol. 7, No. 1, 01.01.2014, p. 124-133.

Research output: Contribution to journalArticle

Leung, JM, Davenport, M, Wolff, MJ, Wiens, KE, Abidi, WM, Poles, MA, Cho, I, Ullman, TA, Mayer, L & Loke, P 2014, 'IL-22-producing CD4+ cells are depleted in actively inflamed colitis tissue', Mucosal Immunology, vol. 7, no. 1, pp. 124-133. https://doi.org/10.1038/mi.2013.31
Leung JM, Davenport M, Wolff MJ, Wiens KE, Abidi WM, Poles MA et al. IL-22-producing CD4+ cells are depleted in actively inflamed colitis tissue. Mucosal Immunology. 2014 Jan 1;7(1):124-133. https://doi.org/10.1038/mi.2013.31
Leung, J. M. ; Davenport, M. ; Wolff, M. J. ; Wiens, K. E. ; Abidi, W. M. ; Poles, M. A. ; Cho, I. ; Ullman, Thomas A. ; Mayer, L. ; Loke, P. / IL-22-producing CD4+ cells are depleted in actively inflamed colitis tissue. In: Mucosal Immunology. 2014 ; Vol. 7, No. 1. pp. 124-133.
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