IL-17 stimulates intraperitoneal neutrophil infiltration through the release of GROα chemokine from mesothelial cells

J. Witowski, K. Pawlaczyk, A. Breborowicz, A. Scheuren, M. Kuzlan-Pawlaczyk, J. Wisniewska, A. Polubinska, H. Friess, G. M. Gahl, U. Frei, A. Jorres

Research output: Contribution to journalArticle

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Abstract

IL-17 is a newly discovered cytokine implicated in the regulation of hemopoiesis and inflammation. Because IL-17 production is restricted to activated T lymphocytes, the effects exerted by IL-17 may help one to understand the contribution of T cells to the inflammatory response. We investigated the role of IL-17 in leukocyte recruitment into the peritoneal cavity. Leukocyte infiltration in vivo was assessed in BALB/Cj mice. Effects of IL-17 on chemokine generation in vitro were examined in human peritoneal mesothelial cells (HPMC). Administration of IL-17 i.p. resulted in a selective recruitment of neutrophils into the peritoneum and increased levels of KC chemokine (murine homologue of human growth-related oncogene α (GROα). Pretreatment with anti-KC Ab significantly reduced the IL-17-driven neutrophil accumulation. Primary cultures of HPMC expressed IL-17 receptor mRNA. Exposure of HPMC to IL-17 led to a dose- and time-dependent induction of GROα mRNA and protein. Combination of IL-17 together with TNF-α resulted in an increased stability of GROα mRNA and synergistic release of GROα protein. Anti-IL-17 Ab blocked the effects of IL-17 in vitro and in vivo. IL-17 is capable of selectively recruiting neutrophils into the peritoneal cavity via the release of neutrophil-specific chemokines from the peritoneal mesothelium.

Original languageEnglish (US)
Pages (from-to)5814-5821
Number of pages8
JournalJournal of Immunology
Volume165
Issue number10
StatePublished - Nov 15 2000
Externally publishedYes

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Interleukin-17
Neutrophil Infiltration
Oncogenes
Chemokines
Growth
Neutrophils
Oncogene Proteins
Peritoneal Cavity
Messenger RNA
Interleukin-17 Receptors
Leukocytes
T-Lymphocytes
Peritoneum
Epithelium

ASJC Scopus subject areas

  • Immunology

Cite this

Witowski, J., Pawlaczyk, K., Breborowicz, A., Scheuren, A., Kuzlan-Pawlaczyk, M., Wisniewska, J., ... Jorres, A. (2000). IL-17 stimulates intraperitoneal neutrophil infiltration through the release of GROα chemokine from mesothelial cells. Journal of Immunology, 165(10), 5814-5821.

IL-17 stimulates intraperitoneal neutrophil infiltration through the release of GROα chemokine from mesothelial cells. / Witowski, J.; Pawlaczyk, K.; Breborowicz, A.; Scheuren, A.; Kuzlan-Pawlaczyk, M.; Wisniewska, J.; Polubinska, A.; Friess, H.; Gahl, G. M.; Frei, U.; Jorres, A.

In: Journal of Immunology, Vol. 165, No. 10, 15.11.2000, p. 5814-5821.

Research output: Contribution to journalArticle

Witowski, J, Pawlaczyk, K, Breborowicz, A, Scheuren, A, Kuzlan-Pawlaczyk, M, Wisniewska, J, Polubinska, A, Friess, H, Gahl, GM, Frei, U & Jorres, A 2000, 'IL-17 stimulates intraperitoneal neutrophil infiltration through the release of GROα chemokine from mesothelial cells', Journal of Immunology, vol. 165, no. 10, pp. 5814-5821.
Witowski J, Pawlaczyk K, Breborowicz A, Scheuren A, Kuzlan-Pawlaczyk M, Wisniewska J et al. IL-17 stimulates intraperitoneal neutrophil infiltration through the release of GROα chemokine from mesothelial cells. Journal of Immunology. 2000 Nov 15;165(10):5814-5821.
Witowski, J. ; Pawlaczyk, K. ; Breborowicz, A. ; Scheuren, A. ; Kuzlan-Pawlaczyk, M. ; Wisniewska, J. ; Polubinska, A. ; Friess, H. ; Gahl, G. M. ; Frei, U. ; Jorres, A. / IL-17 stimulates intraperitoneal neutrophil infiltration through the release of GROα chemokine from mesothelial cells. In: Journal of Immunology. 2000 ; Vol. 165, No. 10. pp. 5814-5821.
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