IL-10 mediates monocyte hyporesponsiveness in septic shock

Tacla Sfeir, Dhanonjoy C. Saha, Mark Astiz, Eric Rackow

Research output: Contribution to journalArticle

Abstract

Introduction: Septic shock is associated with alterations in monocyte function, IL-10 has known immunosuppressive properties. We examined the role of IL-10 as a mediator of monocyte down regulation in septic shock. Methods: Blood mononuclear cells from healthy volunteers (n=10) were isolated and cultured. Culture plate adherent monocytes were incubated with either normal plasma (NP) or septic plasma (SP). Septic plasma was collected from septic shock patients within 24 hours of the diagnosis of sepsis. Following incubation with normal or septic plasma, LPS was added and the monocytes were incubated for 18 hours at 37°C (NPL or SPL). The supernatant was collected and analyzed for tumor necrosis factor (TNF-α), interleukin 1 (IL-1 β) and interleukin 10 (IL-10) by ELISA. In a parallel experiment antibody to IL-10 was added to the monocytes prior to adding the septic plasma and LPS (ASPL). Values are mean ± SEM,*p

Original languageEnglish (US)
JournalCritical Care Medicine
Volume27
Issue number1 SUPPL.
StatePublished - 1999
Externally publishedYes

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Septic Shock
Interleukin-10
Monocytes
Immunosuppressive Agents
Interleukin-1
Blood Cells
Sepsis
Healthy Volunteers
Down-Regulation
Tumor Necrosis Factor-alpha
Enzyme-Linked Immunosorbent Assay
Antibodies

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

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IL-10 mediates monocyte hyporesponsiveness in septic shock. / Sfeir, Tacla; Saha, Dhanonjoy C.; Astiz, Mark; Rackow, Eric.

In: Critical Care Medicine, Vol. 27, No. 1 SUPPL., 1999.

Research output: Contribution to journalArticle

Sfeir, T, Saha, DC, Astiz, M & Rackow, E 1999, 'IL-10 mediates monocyte hyporesponsiveness in septic shock', Critical Care Medicine, vol. 27, no. 1 SUPPL..
Sfeir, Tacla ; Saha, Dhanonjoy C. ; Astiz, Mark ; Rackow, Eric. / IL-10 mediates monocyte hyporesponsiveness in septic shock. In: Critical Care Medicine. 1999 ; Vol. 27, No. 1 SUPPL.
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