IL-10 fails to abrogate experimental autoimmune encephalomyelitis

B. Cannella, Y. L. Gao, C. Brosnan, C. S. Raine

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Mice adoptively-sensitized to develop chronic relapsing experimental autoimmune encephalomyelitis (EAE), a model for the human demyelinating condition, multiple sclerosis (MS), were given injections of recombinant human IL-10 at various timepoints postsensitization in an attempt to abrogate disease development. IL-10 is a Th2 immunomodulatory cytokine with known down-regulatory effects upon Th1 responses and macrophages. Contrary to a previous report on EAE and the predicted outcome, after repeated experiments, IL-10 was found to elicit a worsening or no effect upon EAE in the mouse. Animals were studied clinically, histopathologically and immunocytochemically. On no occasion was disease ameliorated by IL-10. Pretreatment with IL-10 of lymph node cells used to transfer EAE had no effect upon disease outcome, indicating that the cells were already committed effectors. Administration of anti-IL-10 monoclonal antibody before onset of signs had no effect when given early post-sensitization and caused marked worsening when given immediately before onset of signs. In the context of this autoimmune demyelinating model, these results suggest that IL-10 alone is insufficient to reverse the effector response and indeed may serve to enhance the cascade of events in EAE.

Original languageEnglish (US)
Pages (from-to)735-746
Number of pages12
JournalJournal of Neuroscience Research
Volume45
Issue number6
DOIs
StatePublished - Sep 15 1996

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Autoimmune Experimental Encephalomyelitis
Interleukin-10
Multiple Sclerosis
Lymph Nodes
Macrophages
Monoclonal Antibodies
Cytokines
Injections

Keywords

  • autoimmunity
  • cytokines
  • multiple sclerosis
  • myelin
  • neuropathology

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

IL-10 fails to abrogate experimental autoimmune encephalomyelitis. / Cannella, B.; Gao, Y. L.; Brosnan, C.; Raine, C. S.

In: Journal of Neuroscience Research, Vol. 45, No. 6, 15.09.1996, p. 735-746.

Research output: Contribution to journalArticle

Cannella, B. ; Gao, Y. L. ; Brosnan, C. ; Raine, C. S. / IL-10 fails to abrogate experimental autoimmune encephalomyelitis. In: Journal of Neuroscience Research. 1996 ; Vol. 45, No. 6. pp. 735-746.
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