IFN-γ/lipopolysaccharide activation of macrophages is associated with protein kinase C-dependent down-modulation of the colony-stimulating factor-1 receptor

M. Baccarini, P. D. Sbarba, D. Buscher, A. Bartocci, E. R. Stanley

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Abstract

IFNγ/LPS treatment increases macrophage tumoricidal and microbicidal activity and inhibits CSF-1-induced macrophage proliferation. The mechanism underlying the latter effect was investigated in the CSF-1-dependent mouse macrophage cell line, BAC-1.2F5. IFN-γ and LPS together dramatically reduced the total number of CSF-1 receptors (CSF-1R) via selective degradation of the cell surface form. Processing and transport of intracellular CSF-1R to the cell surface were unaffected. IFN-γ alone had no effect but significantly enhanced LPS-induced CSF-1R down-regulation. The reduction in CSF-1R number was protein kinase C-dependent and involved changes in serine phosphorylation of the receptor at different sites. CSF-1R down-modulation by this mechanism may be important in switching off the energy-consuming processes of CSF-1R- mediated proliferation and chemotaxis in activated macrophages.

Original languageEnglish (US)
Pages (from-to)2656-2661
Number of pages6
JournalJournal of Immunology
Volume149
Issue number8
Publication statusPublished - Jan 1 1992

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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