IFN-γ/lipopolysaccharide activation of macrophages is associated with protein kinase C-dependent down-modulation of the colony-stimulating factor-1 receptor

Manuela Baccarini, Persio Dello Sbarba, Dirk Buscher, Anna Bartocci, E. Richard Stanley

Research output: Contribution to journalArticle

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Abstract

IFNγ/LPS treatment increases macrophage tumoricidal and microbicidal activity and inhibits CSF-1-induced macrophage proliferation. The mechanism underlying the latter effect was investigated in the CSF-1-dependent mouse macrophage cell line, BAC-1.2F5. IFN-γ and LPS together dramatically reduced the total number of CSF-1 receptors (CSF-1R) via selective degradation of the cell surface form. Processing and transport of intracellular CSF-1R to the cell surface were unaffected. IFN-γ alone had no effect but significantly enhanced LPS-induced CSF-1R down-regulation. The reduction in CSF-1R number was protein kinase C-dependent and involved changes in serine phosphorylation of the receptor at different sites. CSF-1R down-modulation by this mechanism may be important in switching off the energy-consuming processes of CSF-1R-mediated proliferation and chemotaxis in activated macrophages.

Original languageEnglish (US)
Pages (from-to)2656-2661
Number of pages6
JournalJournal of Immunology
Volume149
Issue number8
StatePublished - Oct 15 1992

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Colony-Stimulating Factor Receptors
Macrophage Colony-Stimulating Factor Receptors
Macrophage Colony-Stimulating Factor
Macrophage Activation
Protein Kinase C
Lipopolysaccharides
Macrophages
Chemotaxis
Down-Regulation
Phosphorylation
Cell Line

ASJC Scopus subject areas

  • Immunology

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IFN-γ/lipopolysaccharide activation of macrophages is associated with protein kinase C-dependent down-modulation of the colony-stimulating factor-1 receptor. / Baccarini, Manuela; Sbarba, Persio Dello; Buscher, Dirk; Bartocci, Anna; Stanley, E. Richard.

In: Journal of Immunology, Vol. 149, No. 8, 15.10.1992, p. 2656-2661.

Research output: Contribution to journalArticle

Baccarini, Manuela ; Sbarba, Persio Dello ; Buscher, Dirk ; Bartocci, Anna ; Stanley, E. Richard. / IFN-γ/lipopolysaccharide activation of macrophages is associated with protein kinase C-dependent down-modulation of the colony-stimulating factor-1 receptor. In: Journal of Immunology. 1992 ; Vol. 149, No. 8. pp. 2656-2661.
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