IFITM3 directly engages and shuttles incoming virus particles to lysosomes

Jennifer S. Spence, Ruina He, Hans Heinrich Hoffmann, Tandrila Das, Emmanuelle Thinon, Charles M. Rice, Tao Peng, Kartik Chandran, Howard C. Hang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Interferon-induced transmembrane proteins (IFITMs 1, 2 and 3) have emerged as important innate immune effectors that prevent diverse virus infections in vertebrates. However, the cellular mechanisms and live-cell imaging of these small membrane proteins have been challenging to evaluate during viral entry of mammalian cells. Using CRISPR–Cas9-mediated IFITM-mutant cell lines, we demonstrate that human IFITM1, IFITM2 and IFITM3 act cooperatively and function in a dose-dependent fashion in interferon-stimulated cells. Through site-specific fluorophore tagging and live-cell imaging studies, we show that IFITM3 is on endocytic vesicles that fuse with incoming virus particles and enhances the trafficking of this pathogenic cargo to lysosomes. IFITM3 trafficking is specific to restricted viruses, requires S-palmitoylation and is abrogated with loss-of-function mutants. The site-specific protein labeling and live-cell imaging approaches described here should facilitate the functional analysis of host factors involved in pathogen restriction as well as their mechanisms of regulation.

Original languageEnglish (US)
JournalNature Chemical Biology
DOIs
StateAccepted/In press - Jan 1 2019

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Lysosomes
Virion
Lipoylation
Transport Vesicles
Virus Diseases
Interferons
Statistical Factor Analysis
Vertebrates
Membrane Proteins
Viruses
Cell Line
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Spence, J. S., He, R., Hoffmann, H. H., Das, T., Thinon, E., Rice, C. M., ... Hang, H. C. (Accepted/In press). IFITM3 directly engages and shuttles incoming virus particles to lysosomes. Nature Chemical Biology. https://doi.org/10.1038/s41589-018-0213-2

IFITM3 directly engages and shuttles incoming virus particles to lysosomes. / Spence, Jennifer S.; He, Ruina; Hoffmann, Hans Heinrich; Das, Tandrila; Thinon, Emmanuelle; Rice, Charles M.; Peng, Tao; Chandran, Kartik; Hang, Howard C.

In: Nature Chemical Biology, 01.01.2019.

Research output: Contribution to journalArticle

Spence, Jennifer S. ; He, Ruina ; Hoffmann, Hans Heinrich ; Das, Tandrila ; Thinon, Emmanuelle ; Rice, Charles M. ; Peng, Tao ; Chandran, Kartik ; Hang, Howard C. / IFITM3 directly engages and shuttles incoming virus particles to lysosomes. In: Nature Chemical Biology. 2019.
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