Identifying Urinary and Serum Exosome Biomarkers for Radiation Exposure Using a Data Dependent Acquisition and SWATH-MS Combined Workflow

Shilpa Kulkarni, Antonius Koller, Kartik M. Mani, Ruofeng Wen, Alan Alfieri, Subhrajit Saha, Jian Wang, Purvi Patel, Nuno Bandeira, Chandan Guha, Emily I. Chen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Purpose Early and accurate assessment of radiation injury by radiation-responsive biomarkers is critical for triage and early intervention. Biofluids such as urine and serum are convenient for such analysis. Recent research has also suggested that exosomes are a reliable source of biomarkers in disease progression. In the present study, we analyzed total urine proteome and exosomes isolated from urine or serum for potential biomarkers of acute and persistent radiation injury in mice exposed to lethal whole body irradiation (WBI). Methods and Materials For feasibility studies, the mice were irradiated at 10.4 Gy WBI, and urine and serum samples were collected 24 and 72 hours after irradiation. Exosomes were isolated and analyzed using liquid chromatography mass spectrometry/mass spectrometry-based workflow for radiation exposure signatures. A data dependent acquisition and SWATH-MS combined workflow approach was used to identify significantly exosome biomarkers indicative of acute or persistent radiation-induced responses. For the validation studies, mice were exposed to 3, 6, 8, or 10 Gy WBI, and samples were analyzed for comparison. Results A comparison between total urine proteomics and urine exosome proteomics demonstrated that exosome proteomic analysis was superior in identifying radiation signatures. Feasibility studies identified 23 biomarkers from urine and 24 biomarkers from serum exosomes after WBI. Urinary exosome signatures identified different physiological parameters than the ones obtained in serum exosomes. Exosome signatures from urine indicated injury to the liver, gastrointestinal, and genitourinary tracts. In contrast, serum showed vascular injuries and acute inflammation in response to radiation. Selected urinary exosomal biomarkers also showed changes at lower radiation doses in validation studies. Conclusions Exosome proteomics revealed radiation- and time-dependent protein signatures after WBI. A total of 47 differentially secreted proteins were identified in urinary and serum exosomes. Together, these data showed the feasibility of defining biomarkers that could elucidate tissue-associated and systemic response caused by high-dose ionizing radiation. This is the first report using an exosome proteomics approach to identify radiation signatures.

Original languageEnglish (US)
Pages (from-to)566-577
Number of pages12
JournalInternational Journal of Radiation Oncology Biology Physics
Volume96
Issue number3
DOIs
StatePublished - Nov 1 2016

Fingerprint

SWATH (ship)
Exosomes
urine
Workflow
biomarkers
radiation dosage
serums
acquisition
Biomarkers
signatures
Serum
radiation
irradiation
Urine
Whole-Body Irradiation
Radiation
Proteomics
radiation injuries
mice
mass spectroscopy

ASJC Scopus subject areas

  • Radiation
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

Identifying Urinary and Serum Exosome Biomarkers for Radiation Exposure Using a Data Dependent Acquisition and SWATH-MS Combined Workflow. / Kulkarni, Shilpa; Koller, Antonius; Mani, Kartik M.; Wen, Ruofeng; Alfieri, Alan; Saha, Subhrajit; Wang, Jian; Patel, Purvi; Bandeira, Nuno; Guha, Chandan; Chen, Emily I.

In: International Journal of Radiation Oncology Biology Physics, Vol. 96, No. 3, 01.11.2016, p. 566-577.

Research output: Contribution to journalArticle

Kulkarni, Shilpa ; Koller, Antonius ; Mani, Kartik M. ; Wen, Ruofeng ; Alfieri, Alan ; Saha, Subhrajit ; Wang, Jian ; Patel, Purvi ; Bandeira, Nuno ; Guha, Chandan ; Chen, Emily I. / Identifying Urinary and Serum Exosome Biomarkers for Radiation Exposure Using a Data Dependent Acquisition and SWATH-MS Combined Workflow. In: International Journal of Radiation Oncology Biology Physics. 2016 ; Vol. 96, No. 3. pp. 566-577.
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AU - Wen, Ruofeng

AU - Alfieri, Alan

AU - Saha, Subhrajit

AU - Wang, Jian

AU - Patel, Purvi

AU - Bandeira, Nuno

AU - Guha, Chandan

AU - Chen, Emily I.

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