Identification of the SL3-3 virus enhancer core as a T-lymphoma cell-specific element

A. L. Boral, S. A. Okenquist, Jack Lenz

Research output: Contribution to journalArticle

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Abstract

Transient expression assays were used to determine the sequences within the long terminal repeat (LTR) that define the high activity in T-lymphoma cells of the leukemogenic SL3-3 virus in comparison with that of the nonleukemogenic Akv virus. Each of these viruses contains sequences related to the consensus element, the enhancer core. The SL3-3 and Akv enhancer cores differ at a single base pair. Substitution of the Akv core element into the SL3-3 LTR decreased expression in T-lymphoma cells but not in other cell types. Likewise, substitution of the SL3-3 core sequence into the Akv LTR increased expression in T-lymphoma cells but not in other types of hematopoietic cells. These data indicate that the SL3-3 enhancer core sequence functions better than that of Akv in T-lymphoma cells, but in other hematopoietic cell types the two are approximately equivalent. Competition DNA-protein binding assays were used to assess what nuclear factors from T-lymphoma lines and non-T lines bound to the SL3-3 and Akv core elements. Factors were detected that bound specifically to either the SL3-3 or Akv core but not to the other. Another factor was detected that bound equally well to both. However, none of these factors was specific to T-lymphoma cells.

Original languageEnglish (US)
Pages (from-to)76-84
Number of pages9
JournalJournal of Virology
Volume63
Issue number1
StatePublished - 1989

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T-Cell Lymphoma
lymphoma
Terminal Repeat Sequences
Viruses
viruses
terminal repeat sequences
cells
DNA-Binding Proteins
Base Pairing
Lymphoma
enhancer elements
DNA-binding proteins
assays

ASJC Scopus subject areas

  • Immunology

Cite this

Identification of the SL3-3 virus enhancer core as a T-lymphoma cell-specific element. / Boral, A. L.; Okenquist, S. A.; Lenz, Jack.

In: Journal of Virology, Vol. 63, No. 1, 1989, p. 76-84.

Research output: Contribution to journalArticle

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AB - Transient expression assays were used to determine the sequences within the long terminal repeat (LTR) that define the high activity in T-lymphoma cells of the leukemogenic SL3-3 virus in comparison with that of the nonleukemogenic Akv virus. Each of these viruses contains sequences related to the consensus element, the enhancer core. The SL3-3 and Akv enhancer cores differ at a single base pair. Substitution of the Akv core element into the SL3-3 LTR decreased expression in T-lymphoma cells but not in other cell types. Likewise, substitution of the SL3-3 core sequence into the Akv LTR increased expression in T-lymphoma cells but not in other types of hematopoietic cells. These data indicate that the SL3-3 enhancer core sequence functions better than that of Akv in T-lymphoma cells, but in other hematopoietic cell types the two are approximately equivalent. Competition DNA-protein binding assays were used to assess what nuclear factors from T-lymphoma lines and non-T lines bound to the SL3-3 and Akv core elements. Factors were detected that bound specifically to either the SL3-3 or Akv core but not to the other. Another factor was detected that bound equally well to both. However, none of these factors was specific to T-lymphoma cells.

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