Identification of the rab5 binding site in p110β

Assays for pi3kβ binding to rab5

Rachel S. Salamon, Hashem A. Dbouk, Denise Collado, Jaclyn Lopiccolo, Anne R. Bresnick, Jonathan M. Backer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Isoform-specific signaling by Class IA PI 3-kinases depends in part on the interactions between distinct catalytic subunits and upstream regulatory proteins. From among the class IA catalytic subunits (p110α, p110β, and p110δ), p110β has unique properties. Unlike the other family members, p110β directly binds to Gβγ subunits, downstream from activated G-protein coupled receptors, and to activated Rab5. Furthermore, the Ras-binding domain (RBD) of p110β binds to Rac and Cdc42 but not to Ras. Defining mutations that specifically disrupt these regulatory interactions is critical for defining their role in p110β signaling. This chapter describes the approach that was used to identify the Rab5 binding site in p110β, and discusses methods for the analysis of p110β-Rab5 interactions.

Original languageEnglish (US)
Pages (from-to)271-281
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume1298
DOIs
StatePublished - 2015
Externally publishedYes

Fingerprint

Catalytic Domain
Binding Sites
G-Protein-Coupled Receptors
Phosphatidylinositol 3-Kinases
Protein Isoforms
Mutation
Proteins
IA 3

Keywords

  • Class IA PI 3-kinase
  • Lipid kinases
  • P110beta
  • Phosphoinositide 3-kinases
  • PIK3CB
  • Rab5
  • Small GTPases

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Identification of the rab5 binding site in p110β : Assays for pi3kβ binding to rab5. / Salamon, Rachel S.; Dbouk, Hashem A.; Collado, Denise; Lopiccolo, Jaclyn; Bresnick, Anne R.; Backer, Jonathan M.

In: Methods in molecular biology (Clifton, N.J.), Vol. 1298, 2015, p. 271-281.

Research output: Contribution to journalArticle

Salamon, Rachel S. ; Dbouk, Hashem A. ; Collado, Denise ; Lopiccolo, Jaclyn ; Bresnick, Anne R. ; Backer, Jonathan M. / Identification of the rab5 binding site in p110β : Assays for pi3kβ binding to rab5. In: Methods in molecular biology (Clifton, N.J.). 2015 ; Vol. 1298. pp. 271-281.
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