Identification of the GTP-binding protein encoded by Gi3 complementary DNA.

P. Goldsmith, K. Rossiter, A. Carter, W. Simonds, C. G. Unson, R. Vinitsky, A. M. Spiegel

Research output: Contribution to journalArticle

124 Scopus citations

Abstract

Three closely related, but distinct, GTP-binding proteins (G-proteins) are encoded by cDNAs arbitrarily designated Gi1, Gi2, and Gi3. The in vitro translated products of mRNAs prepared from Gi1, Gi2, and Gi3 cDNAs migrate as 41-, 40-, and 41-kDa proteins, respectively, on sodium dodecyl sulfate-polyacrylamide gels. Antisera were raised against synthetic decapeptides corresponding to a divergent sequence (residues 159-168 for Gi1 and Gi3; 160-169 for Gi2) of the three cDNAs and tested on immunoblots for reactivity with three purified G-proteins, G41 and G40 from brain and G41 from HL-60 cells. LD antisera (Gi1 peptide) react only with brain G41. LE antisera (Gi2 peptide) react only with brain G40, and SQ antisera (Gi3 peptide) react exclusively with HL-60 G41. The results indicate that the 41-kDa G-protein purified from HL-60 cells differs from the purified brain 41-kDa protein and suggest that the HL-60 cell protein corresponds to that encoded by Gi3 cDNA.

Original languageEnglish (US)
Pages (from-to)6476-6479
Number of pages4
JournalThe Journal of biological chemistry
Volume263
Issue number14
StatePublished - May 15 1988
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Goldsmith, P., Rossiter, K., Carter, A., Simonds, W., Unson, C. G., Vinitsky, R., & Spiegel, A. M. (1988). Identification of the GTP-binding protein encoded by Gi3 complementary DNA. The Journal of biological chemistry, 263(14), 6476-6479.