Abstract
Purpose We determined whether Raman spectroscopy could identify spermatogenesis in a Sertoli-cell only rat model. Materials and Methods A partial Sertoli-cell only model was created using a testicular hypothermia-ischemia technique. Bilateral testis biopsy was performed in 4 rats. Raman spectra were acquired with a probe in 1 mm3 samples of testicular tissue. India ink was used to mark the site of spectral acquisition. Comparative histopathology was applied to verify whether Raman spectra were obtained from Sertoli-cell only tubules or seminiferous tubules with spermatogenesis. Principal component analysis and logistic regression were used to develop a mathematical model to evaluate the predictive accuracy of identifying tubules with spermatogenesis vs Sertoli-cell only tubules. Results Raman peak intensity changes were noted at 1,000 and 1,690 cm-1 for tubules with spermatogenesis and Sertoli-cell only tubules, respectively. When principal components were used to predict whether seminferous tubules were Sertoli-cell only tubules or showed spermatogenesis, sensitivity and specificity were 96% and 100%, respectively. The ROC AUC to predict tubules with spermatogenesis with Raman spectroscopy was 0.98. Conclusions Raman spectroscopy is capable of identifying seminiferous tubules with spermatogenesis in a Sertoli-cell only ex vivo rat model. Future ex vivo studies of human testicular tissue are necessary to confirm whether these findings can be translated to the clinical setting.
Original language | English (US) |
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Pages (from-to) | 607-612 |
Number of pages | 6 |
Journal | Journal of Urology |
Volume | 192 |
Issue number | 2 |
DOIs | |
State | Published - Aug 2014 |
Externally published | Yes |
Keywords
- Raman
- Sertoli cell-only syndrome
- azoospermia
- spectrum analysis
- spermatogenesis
- testis
ASJC Scopus subject areas
- Urology