Identification of regulatory elements within the minimal promoter region of the human endogenous ERV9 proviruses: Accurate transcription initiation is controlled by an Inr-like element

Girolama La Mantia, Barbara Majello, Antonio Di Cristofano, Maria Strazzullo, Gabriella Minchiotti, Luigi Lania

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

ERV9 is a low repeated family of human endogenous retroviral elements whose expression is mainly detectable in undifferentiated embryonal carcinoma NT2/01 cells. In this report we have analyzed the minimal promoter region located within the ERV9 LTR. Using the transient CAT expression assay we have identified the minimal promoter region, which includes sequences spanning from -70 to +6 relative to the major transcription start site. Deletion analysis, primer extension mapping of the transcription start sites and DNA-protein interactions assays have allowed us to define two important regions within the ERV9 minimal promoter. One region located between -70 to -39 acts as a transcriptional activating sequence and contains an Sp1 binding site. The second region from -7 to +6, which resembles an initiator element (Inr), was necessary for the correct transcription start site utilization, and binds to a regulatory protein. Cross-competition experiments using various lnr elements have indicated that the protein that binds to the ERV9 lnr element can be competed by the HIV-1 and TdT Inr sequences.

Original languageEnglish (US)
Pages (from-to)4129-4136
Number of pages8
JournalNucleic Acids Research
Volume20
Issue number16
StatePublished - Aug 25 1992
Externally publishedYes

Fingerprint

Proviruses
Transcription Initiation Site
Transcription
Genetic Promoter Regions
Promoter
Proteins
Assays
Embryonal Carcinoma
Protein
Binding sites
HIV-1
DNA
Binding Sites
Carcinoma
Deletion
Human
Experiments
Necessary
Cell
Interaction

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Identification of regulatory elements within the minimal promoter region of the human endogenous ERV9 proviruses : Accurate transcription initiation is controlled by an Inr-like element. / La Mantia, Girolama; Majello, Barbara; Di Cristofano, Antonio; Strazzullo, Maria; Minchiotti, Gabriella; Lania, Luigi.

In: Nucleic Acids Research, Vol. 20, No. 16, 25.08.1992, p. 4129-4136.

Research output: Contribution to journalArticle

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