@article{873ee25243174823a3a16465314f0171,
title = "Identification of rare immunoglobulin switch variants using the ELISA spot assay",
abstract = "We describe here the use of the ELISA spot assay to identify, quantify, and isolate rare hybridoma subclones that have switched to expressing a new class or subclass of Ig. This technique is less labor intensive and time consuming than sib selection and standard ELISA and eliminates the many false positives that complicated those techniques. The use of the ELISA spot assay also allows screening large populations of cells and accurate quantitation of the rate of isotype switching.",
keywords = "Class and subclass switch, ELISA spot assay, Monoclonal antibody",
author = "Gadi Spira and Scharff, {Matthew D.}",
note = "Funding Information: We would like to thank PeiYing Yang for her technical assistance, Dr. L. Zuckier and Dr. T.V. Rajan for advising on the fluctuation analysis, Nancy Drenzyk for preparing this manuscript, and Dr. M. Paizi for helping in the preparation of the figures. This study was carried out in the Hybridoma Facility of the Cancer Center and supported by grants from the United States-Israel Binational Research Fund (8900310) and the National Institute of Health (CA39838, POICA1330). Dr. Scharff is supported in part by the Harry Eagle Chair for Cancer Research from the National Women's Division of the Albert Einstein College of Medicine.",
year = "1992",
doi = "10.1016/0022-1759(92)90165-P",
language = "English (US)",
volume = "148",
pages = "121--129",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1-2",
}