Identification of rare immunoglobulin switch variants using the ELISA spot assay

Gadi Spira, Matthew D. Scharff

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

We describe here the use of the ELISA spot assay to identify, quantify, and isolate rare hybridoma subclones that have switched to expressing a new class or subclass of Ig. This technique is less labor intensive and time consuming than sib selection and standard ELISA and eliminates the many false positives that complicated those techniques. The use of the ELISA spot assay also allows screening large populations of cells and accurate quantitation of the rate of isotype switching.

Original languageEnglish (US)
Pages (from-to)121-129
Number of pages9
JournalJournal of Immunological Methods
Volume148
Issue number1-2
DOIs
Publication statusPublished - 1992

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Keywords

  • Class and subclass switch
  • ELISA spot assay
  • Monoclonal antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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