Identification of Proton NMR Signals from the Metal Ligands in Cadmium-Substituted Plastocyanin via Two-Dimensional Multiple-Quantum Detection in the Absence of Explicitly Resolved 1H-113Cd Coupling

David H. Live, Christopher L. Kojiro, David Cowburn, John L. Markley

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Two-dimensional (2D) proton-detected heteronuclear 1HP{113Cd} multiple-quantum (HMQ) NMR spectroscopy of 113Cd-substituted plastocyanin from spinach (Spinacia oleracea) yielded signals from the four amino acid side chains ligated to the metal. HMQ coherences over four bonds were observed with the metal ligands histidine-37 and histidine-87. Assignments of signals from the metal ligands, cysteine-84 Cβ-1H2 and methionine-92 C-1H3, are proposed. HMQ signals were observed in the absence of resolved 1H-113Cd coupling. The 2D HMQ method should be useful for identifying !H NMR peaks from metal ligands in metalloproteins.

Original languageEnglish (US)
Pages (from-to)3043-3045
Number of pages3
JournalJournal of the American Chemical Society
Volume107
Issue number11
DOIs
Publication statusPublished - May 1985
Externally publishedYes

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ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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