Identification of Proton NMR Signals from the Metal Ligands in Cadmium-Substituted Plastocyanin via Two-Dimensional Multiple-Quantum Detection in the Absence of Explicitly Resolved 1H-113Cd Coupling

David H. Live; Christopher L. Kojiro; David Cowburn; John L. Markley

doi:10.1021/ja00297a008

Identification of Proton NMR Signals from the Metal Ligands in Cadmium-Substituted Plastocyanin via Two-Dimensional Multiple-Quantum Detection in the Absence of Explicitly Resolved ¹H-¹¹³Cd Coupling

David H. Live, Christopher L. Kojiro, David Cowburn, John L. Markley

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Two-dimensional (2D) proton-detected heteronuclear ¹HP{¹¹³Cd} multiple-quantum (HMQ) NMR spectroscopy of ¹¹³Cd-substituted plastocyanin from spinach (Spinacia oleracea) yielded signals from the four amino acid side chains ligated to the metal. HMQ coherences over four bonds were observed with the metal ligands histidine-37 and histidine-87. Assignments of signals from the metal ligands, cysteine-84 C_β-¹H₂ and methionine-92 C_∊-¹H₃, are proposed. HMQ signals were observed in the absence of resolved ¹H-¹¹³Cd coupling. The 2D HMQ method should be useful for identifying ^!H NMR peaks from metal ligands in metalloproteins.

Original language	English (US)
Pages (from-to)	3043-3045
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	107
Issue number	11
DOIs	https://doi.org/10.1021/ja00297a008
State	Published - May 1985
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja00297a008

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Identification of Proton NMR Signals from the Metal Ligands in Cadmium-Substituted Plastocyanin via Two-Dimensional Multiple-Quantum Detection in the Absence of Explicitly Resolved ¹H-¹¹³Cd Coupling. / Live, David H.; Kojiro, Christopher L.; Cowburn, David et al.
In: Journal of the American Chemical Society, Vol. 107, No. 11, 05.1985, p. 3043-3045.

Research output: Contribution to journal › Article › peer-review

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abstract = "Two-dimensional (2D) proton-detected heteronuclear 1HP{113Cd} multiple-quantum (HMQ) NMR spectroscopy of 113Cd-substituted plastocyanin from spinach (Spinacia oleracea) yielded signals from the four amino acid side chains ligated to the metal. HMQ coherences over four bonds were observed with the metal ligands histidine-37 and histidine-87. Assignments of signals from the metal ligands, cysteine-84 Cβ-1H2 and methionine-92 C∊-1H3, are proposed. HMQ signals were observed in the absence of resolved 1H-113Cd coupling. The 2D HMQ method should be useful for identifying !H NMR peaks from metal ligands in metalloproteins.",

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AU - Kojiro, Christopher L.

AU - Cowburn, David

AU - Markley, John L.

PY - 1985/5

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N2 - Two-dimensional (2D) proton-detected heteronuclear 1HP{113Cd} multiple-quantum (HMQ) NMR spectroscopy of 113Cd-substituted plastocyanin from spinach (Spinacia oleracea) yielded signals from the four amino acid side chains ligated to the metal. HMQ coherences over four bonds were observed with the metal ligands histidine-37 and histidine-87. Assignments of signals from the metal ligands, cysteine-84 Cβ-1H2 and methionine-92 C∊-1H3, are proposed. HMQ signals were observed in the absence of resolved 1H-113Cd coupling. The 2D HMQ method should be useful for identifying !H NMR peaks from metal ligands in metalloproteins.

AB - Two-dimensional (2D) proton-detected heteronuclear 1HP{113Cd} multiple-quantum (HMQ) NMR spectroscopy of 113Cd-substituted plastocyanin from spinach (Spinacia oleracea) yielded signals from the four amino acid side chains ligated to the metal. HMQ coherences over four bonds were observed with the metal ligands histidine-37 and histidine-87. Assignments of signals from the metal ligands, cysteine-84 Cβ-1H2 and methionine-92 C∊-1H3, are proposed. HMQ signals were observed in the absence of resolved 1H-113Cd coupling. The 2D HMQ method should be useful for identifying !H NMR peaks from metal ligands in metalloproteins.

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Identification of Proton NMR Signals from the Metal Ligands in Cadmium-Substituted Plastocyanin via Two-Dimensional Multiple-Quantum Detection in the Absence of Explicitly Resolved ¹H-¹¹³Cd Coupling

Abstract

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