Identification of proton NMR signals from the metal ligands in cadmium-substituted plastocyanin via two-dimensional multiple-quantum detection in the absence of explicitly resolved 1H-113Cd coupling

David H. Live, Christopher L. Kojiro, David Cowburn, John L. Markley

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26 Citations (Scopus)

Abstract

Two-dimensional (2D) proton-detected heteronuclear 1H{113Cd} multiple-quantum (HMQ) NMR spectroscopy of 113Cd-substituted plastocyanin from spinach (Spinacia oleracea) yielded signals from the four amino acid side chains ligated to the metal. HMQ coherences over four bonds were observed with the metal ligands histidine-37 and histidine-87. Assignments of signals from the metal ligands, cysteine-84 Cβ-1H2 and methionine-92 Cε-1H3, are proposed. HMQ signals were observed in the absence of resolved 1H-113Cd coupling. The 2D HMQ method should be useful for identifying 1H NMR peaks from metal ligands in metalloproteins.

Original languageEnglish (US)
Pages (from-to)3043-3045
Number of pages3
JournalJournal of the American Chemical Society
Volume107
Issue number11
StatePublished - 1985
Externally publishedYes

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Plastocyanin
Cadmium
Protons
Metals
Ligands
Nuclear magnetic resonance
Spinacia oleracea
Histidine
Metalloproteins
Methionine
Nuclear magnetic resonance spectroscopy
Cysteine
Amino acids
Magnetic Resonance Spectroscopy
Amino Acids

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

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title = "Identification of proton NMR signals from the metal ligands in cadmium-substituted plastocyanin via two-dimensional multiple-quantum detection in the absence of explicitly resolved 1H-113Cd coupling",
abstract = "Two-dimensional (2D) proton-detected heteronuclear 1H{113Cd} multiple-quantum (HMQ) NMR spectroscopy of 113Cd-substituted plastocyanin from spinach (Spinacia oleracea) yielded signals from the four amino acid side chains ligated to the metal. HMQ coherences over four bonds were observed with the metal ligands histidine-37 and histidine-87. Assignments of signals from the metal ligands, cysteine-84 Cβ-1H2 and methionine-92 Cε-1H3, are proposed. HMQ signals were observed in the absence of resolved 1H-113Cd coupling. The 2D HMQ method should be useful for identifying 1H NMR peaks from metal ligands in metalloproteins.",
author = "Live, {David H.} and Kojiro, {Christopher L.} and David Cowburn and Markley, {John L.}",
year = "1985",
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journal = "Journal of the American Chemical Society",
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T1 - Identification of proton NMR signals from the metal ligands in cadmium-substituted plastocyanin via two-dimensional multiple-quantum detection in the absence of explicitly resolved 1H-113Cd coupling

AU - Live, David H.

AU - Kojiro, Christopher L.

AU - Cowburn, David

AU - Markley, John L.

PY - 1985

Y1 - 1985

N2 - Two-dimensional (2D) proton-detected heteronuclear 1H{113Cd} multiple-quantum (HMQ) NMR spectroscopy of 113Cd-substituted plastocyanin from spinach (Spinacia oleracea) yielded signals from the four amino acid side chains ligated to the metal. HMQ coherences over four bonds were observed with the metal ligands histidine-37 and histidine-87. Assignments of signals from the metal ligands, cysteine-84 Cβ-1H2 and methionine-92 Cε-1H3, are proposed. HMQ signals were observed in the absence of resolved 1H-113Cd coupling. The 2D HMQ method should be useful for identifying 1H NMR peaks from metal ligands in metalloproteins.

AB - Two-dimensional (2D) proton-detected heteronuclear 1H{113Cd} multiple-quantum (HMQ) NMR spectroscopy of 113Cd-substituted plastocyanin from spinach (Spinacia oleracea) yielded signals from the four amino acid side chains ligated to the metal. HMQ coherences over four bonds were observed with the metal ligands histidine-37 and histidine-87. Assignments of signals from the metal ligands, cysteine-84 Cβ-1H2 and methionine-92 Cε-1H3, are proposed. HMQ signals were observed in the absence of resolved 1H-113Cd coupling. The 2D HMQ method should be useful for identifying 1H NMR peaks from metal ligands in metalloproteins.

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