Identification of peroxiredoxin-1 as a novel biomarker of abdominal aortic aneurysm

Roxana Martinez-Pinna, Priscila Ramos-Mozo, Julio Madrigal-Matute, Luis M. Blanco-Colio, Juan A. Lopez, Enrique Calvo, Emilio Camafeita, Jes S. Lindholt, Olivier Meilhac, Sandrine Delbosc, Jean Baptiste Michel, Melina Vega De Ceniga, Jesus Egido, Jose L. Martin-Ventura

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Objective- In the search of novel biomarkers of abdominal aortic aneurysm (AAA) progression, proteins released by intraluminal thrombus (ILT) were analyzed by a differential proteomic approach. Methods and Results- Different layers (luminal/abluminal) of the ILT of AAA were incubated, and the proteins released were analyzed by 2-dimensional difference in-gel electrophoresis. Several differentially expressed proteins involved in main AAA pathological mechanisms (proteolysis, oxidative stress, and thrombosis) were identified by mass spectrometry. Among the proteins identified, peroxiredoxin-1 (PRX-1) was more released by the luminal layer compared with the abluminal layer of the ILT, which was further validated by Western blot, ELISA, and immunohistochemistry. We demonstrated increased PRX-1 serum levels in AAA patients compared with healthy subjects and also positive correlation among PRX-1 and AAA diameter, plasmin-antiplasmin, and myeloperoxidase levels. Finally, a prospective study revealed a positive correlation between PRX-1 serum levels and AAA expansion rate. Moreover, the combination of PRX-1 and AAA size had significantly additive value in predicting growth. Conclusion- Several proteins associated with AAA pathogenesis have been identified by a proteomic approach in ILT-conditioned medium. Among them, PRX-1 serum levels are increased in AAA patients and correlate with AAA size and growth rate, suggesting the potential use of PRX-1 as a biomarker for AAA evolution.

Original languageEnglish (US)
Pages (from-to)935-943
Number of pages9
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume31
Issue number4
DOIs
StatePublished - Apr 1 2011
Externally publishedYes

Fingerprint

Peroxiredoxins
Abdominal Aortic Aneurysm
Biomarkers
Thrombosis
Proteins
Proteomics
Serum
Two-Dimensional Difference Gel Electrophoresis
Far-Western Blotting
Antifibrinolytic Agents
Fibrinolysin
Conditioned Culture Medium
Growth
Peroxidase
Proteolysis
Mass Spectrometry
Healthy Volunteers
Oxidative Stress
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry

Keywords

  • aneurysms
  • antioxidants
  • proteomics

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Martinez-Pinna, R., Ramos-Mozo, P., Madrigal-Matute, J., Blanco-Colio, L. M., Lopez, J. A., Calvo, E., ... Martin-Ventura, J. L. (2011). Identification of peroxiredoxin-1 as a novel biomarker of abdominal aortic aneurysm. Arteriosclerosis, Thrombosis, and Vascular Biology, 31(4), 935-943. https://doi.org/10.1161/ATVBAHA.110.214429

Identification of peroxiredoxin-1 as a novel biomarker of abdominal aortic aneurysm. / Martinez-Pinna, Roxana; Ramos-Mozo, Priscila; Madrigal-Matute, Julio; Blanco-Colio, Luis M.; Lopez, Juan A.; Calvo, Enrique; Camafeita, Emilio; Lindholt, Jes S.; Meilhac, Olivier; Delbosc, Sandrine; Michel, Jean Baptiste; De Ceniga, Melina Vega; Egido, Jesus; Martin-Ventura, Jose L.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 31, No. 4, 01.04.2011, p. 935-943.

Research output: Contribution to journalArticle

Martinez-Pinna, R, Ramos-Mozo, P, Madrigal-Matute, J, Blanco-Colio, LM, Lopez, JA, Calvo, E, Camafeita, E, Lindholt, JS, Meilhac, O, Delbosc, S, Michel, JB, De Ceniga, MV, Egido, J & Martin-Ventura, JL 2011, 'Identification of peroxiredoxin-1 as a novel biomarker of abdominal aortic aneurysm', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 31, no. 4, pp. 935-943. https://doi.org/10.1161/ATVBAHA.110.214429
Martinez-Pinna, Roxana ; Ramos-Mozo, Priscila ; Madrigal-Matute, Julio ; Blanco-Colio, Luis M. ; Lopez, Juan A. ; Calvo, Enrique ; Camafeita, Emilio ; Lindholt, Jes S. ; Meilhac, Olivier ; Delbosc, Sandrine ; Michel, Jean Baptiste ; De Ceniga, Melina Vega ; Egido, Jesus ; Martin-Ventura, Jose L. / Identification of peroxiredoxin-1 as a novel biomarker of abdominal aortic aneurysm. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2011 ; Vol. 31, No. 4. pp. 935-943.
@article{1aea743bc0c04a15b02c5279d34740e0,
title = "Identification of peroxiredoxin-1 as a novel biomarker of abdominal aortic aneurysm",
abstract = "Objective- In the search of novel biomarkers of abdominal aortic aneurysm (AAA) progression, proteins released by intraluminal thrombus (ILT) were analyzed by a differential proteomic approach. Methods and Results- Different layers (luminal/abluminal) of the ILT of AAA were incubated, and the proteins released were analyzed by 2-dimensional difference in-gel electrophoresis. Several differentially expressed proteins involved in main AAA pathological mechanisms (proteolysis, oxidative stress, and thrombosis) were identified by mass spectrometry. Among the proteins identified, peroxiredoxin-1 (PRX-1) was more released by the luminal layer compared with the abluminal layer of the ILT, which was further validated by Western blot, ELISA, and immunohistochemistry. We demonstrated increased PRX-1 serum levels in AAA patients compared with healthy subjects and also positive correlation among PRX-1 and AAA diameter, plasmin-antiplasmin, and myeloperoxidase levels. Finally, a prospective study revealed a positive correlation between PRX-1 serum levels and AAA expansion rate. Moreover, the combination of PRX-1 and AAA size had significantly additive value in predicting growth. Conclusion- Several proteins associated with AAA pathogenesis have been identified by a proteomic approach in ILT-conditioned medium. Among them, PRX-1 serum levels are increased in AAA patients and correlate with AAA size and growth rate, suggesting the potential use of PRX-1 as a biomarker for AAA evolution.",
keywords = "aneurysms, antioxidants, proteomics",
author = "Roxana Martinez-Pinna and Priscila Ramos-Mozo and Julio Madrigal-Matute and Blanco-Colio, {Luis M.} and Lopez, {Juan A.} and Enrique Calvo and Emilio Camafeita and Lindholt, {Jes S.} and Olivier Meilhac and Sandrine Delbosc and Michel, {Jean Baptiste} and {De Ceniga}, {Melina Vega} and Jesus Egido and Martin-Ventura, {Jose L.}",
year = "2011",
month = "4",
day = "1",
doi = "10.1161/ATVBAHA.110.214429",
language = "English (US)",
volume = "31",
pages = "935--943",
journal = "Arteriosclerosis, Thrombosis, and Vascular Biology",
issn = "1079-5642",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Identification of peroxiredoxin-1 as a novel biomarker of abdominal aortic aneurysm

AU - Martinez-Pinna, Roxana

AU - Ramos-Mozo, Priscila

AU - Madrigal-Matute, Julio

AU - Blanco-Colio, Luis M.

AU - Lopez, Juan A.

AU - Calvo, Enrique

AU - Camafeita, Emilio

AU - Lindholt, Jes S.

AU - Meilhac, Olivier

AU - Delbosc, Sandrine

AU - Michel, Jean Baptiste

AU - De Ceniga, Melina Vega

AU - Egido, Jesus

AU - Martin-Ventura, Jose L.

PY - 2011/4/1

Y1 - 2011/4/1

N2 - Objective- In the search of novel biomarkers of abdominal aortic aneurysm (AAA) progression, proteins released by intraluminal thrombus (ILT) were analyzed by a differential proteomic approach. Methods and Results- Different layers (luminal/abluminal) of the ILT of AAA were incubated, and the proteins released were analyzed by 2-dimensional difference in-gel electrophoresis. Several differentially expressed proteins involved in main AAA pathological mechanisms (proteolysis, oxidative stress, and thrombosis) were identified by mass spectrometry. Among the proteins identified, peroxiredoxin-1 (PRX-1) was more released by the luminal layer compared with the abluminal layer of the ILT, which was further validated by Western blot, ELISA, and immunohistochemistry. We demonstrated increased PRX-1 serum levels in AAA patients compared with healthy subjects and also positive correlation among PRX-1 and AAA diameter, plasmin-antiplasmin, and myeloperoxidase levels. Finally, a prospective study revealed a positive correlation between PRX-1 serum levels and AAA expansion rate. Moreover, the combination of PRX-1 and AAA size had significantly additive value in predicting growth. Conclusion- Several proteins associated with AAA pathogenesis have been identified by a proteomic approach in ILT-conditioned medium. Among them, PRX-1 serum levels are increased in AAA patients and correlate with AAA size and growth rate, suggesting the potential use of PRX-1 as a biomarker for AAA evolution.

AB - Objective- In the search of novel biomarkers of abdominal aortic aneurysm (AAA) progression, proteins released by intraluminal thrombus (ILT) were analyzed by a differential proteomic approach. Methods and Results- Different layers (luminal/abluminal) of the ILT of AAA were incubated, and the proteins released were analyzed by 2-dimensional difference in-gel electrophoresis. Several differentially expressed proteins involved in main AAA pathological mechanisms (proteolysis, oxidative stress, and thrombosis) were identified by mass spectrometry. Among the proteins identified, peroxiredoxin-1 (PRX-1) was more released by the luminal layer compared with the abluminal layer of the ILT, which was further validated by Western blot, ELISA, and immunohistochemistry. We demonstrated increased PRX-1 serum levels in AAA patients compared with healthy subjects and also positive correlation among PRX-1 and AAA diameter, plasmin-antiplasmin, and myeloperoxidase levels. Finally, a prospective study revealed a positive correlation between PRX-1 serum levels and AAA expansion rate. Moreover, the combination of PRX-1 and AAA size had significantly additive value in predicting growth. Conclusion- Several proteins associated with AAA pathogenesis have been identified by a proteomic approach in ILT-conditioned medium. Among them, PRX-1 serum levels are increased in AAA patients and correlate with AAA size and growth rate, suggesting the potential use of PRX-1 as a biomarker for AAA evolution.

KW - aneurysms

KW - antioxidants

KW - proteomics

UR - http://www.scopus.com/inward/record.url?scp=79953737527&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79953737527&partnerID=8YFLogxK

U2 - 10.1161/ATVBAHA.110.214429

DO - 10.1161/ATVBAHA.110.214429

M3 - Article

C2 - 21273562

AN - SCOPUS:79953737527

VL - 31

SP - 935

EP - 943

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

SN - 1079-5642

IS - 4

ER -