Identification of novel mycobacterial targets for murine CD4+ T-cells by IFNγ ELISPOT

Alison J. Johnson, Steven C. Kennedy, Tony W. Ng, Steven A. Porcelli

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Enzyme-linked immunospot (ELISPOT) is an assay used to detect secretion of cytokines from immune cells. The resolution and sensitivity of ELISPOT allow for the detection of rare T cell specificities and small quantities of molecules produced by individual cells. In this chapter, we describe an epitope screening method that uses CD4+ T cell ELISPOT assays to identify specific novel mycobacterial antigens as potential vaccine candidates. In order to screen a large number of candidate epitopes simultaneously, pools of predicted MHC class II peptides were used to identify mycobacterial specific CD4+ T cells. Using this method, we identified novel mycobacterial antigens as vaccine candidates.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages143-150
Number of pages8
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1808
ISSN (Print)1064-3745

Keywords

  • CD4 T cell
  • Cytokine
  • ELISPOT
  • Epitope
  • Mycobacteria

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Johnson, A. J., Kennedy, S. C., Ng, T. W., & Porcelli, S. A. (2018). Identification of novel mycobacterial targets for murine CD4+ T-cells by IFNγ ELISPOT. In Methods in Molecular Biology (pp. 143-150). (Methods in Molecular Biology; Vol. 1808). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8567-8_12