Identification of new human origins of DNA replication by an origin-trapping assay

Jeannine Gerhardt, Samira Jafar, Mark Peter Spindler, Elisabeth Ott, Aloys Schepers

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Metazoan genomes contain thousands of replication origins, but only a limited number have been characterized so far. We developed a two-step origin-trapping assay in which human chromatin fragments associated with origin recognition complex (ORC) in vivo were first enriched by chromatin immunoprecipitation. In a second step, these fragments were screened for transient replication competence in a plasmid-based assay utilizing the Epstein-Barr virus latent origin oriP. oriP contains two elements, an origin (dyad symmetry element [DS]) and the family of repeats, that when associated with the viral protein EBNA1 facilitate extrachromosomal stability. Insertion of the ORC-binding human DNA fragments in oriP plasmids in place of DS enabled us to screen functionally for their abilities to restore replication. Using the origin-trapping assay, we isolated and characterized five previously unknown human origins. The assay was validated with nascent strand abundance assays that confirm these origins as active initiation sites in their native chromosomal contexts. Furthermore, ORC and MCM2-7 components localized at these origins during G1 phase of the cell cycle but were not detected during mitosis. This finding extends the current understanding of origin-ORC dynamics by suggesting that replication origins must be reestablished during the early stages of each cell division cycle and that ORC itself participates in this process.

Original languageEnglish (US)
Pages (from-to)7731-7746
Number of pages16
JournalMolecular and cellular biology
Volume26
Issue number20
DOIs
StatePublished - Oct 1 2006

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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