Identification of mutant monoclonal antibodies with increased antigen binding

R. R. Pollock, D. L. French, M. L. Gefter, M. D. Scharff

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Sib selection and an ELISA have been used to isolate hybridoma subclones producing mutant antibodies that bind antigen better than the parental monoclonal antibody. Such mutants arise spontaneously in culture at frequencies of 2.5-5 x 10-5. The sequences of the heavy and light chain variable regions of the mutant antibodies are identical to that of the parent and the K(a) values of the mutants and the parent are the same. The increase in binding is associated with abnormalities of the constant region polypeptide and probably reflect changes in avidity of these antibodies.

Original languageEnglish (US)
Pages (from-to)2298-2302
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number7
DOIs
Publication statusPublished - Dec 1 1988

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Keywords

  • avidity
  • hybridomas
  • somatic mutation

ASJC Scopus subject areas

  • General

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