Identification of MEN1 mutations in sporadic enteropancreatic neuroendocrine tumors by analysis of paraffin-embedded tissue

Matthew D. Mailman, Peter Muscarella, William J. Schirmer, E. Christopher Ellison, Thomas M. O'Dorisio, Thomas W. Prior

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Gastrinomas and other gastrointestinal neuroendocrine tumors may occur sporadically or as part of the inherited syndrome multiple endocrine neoplasia type 1 (MEN1). Mutations in the recently identified MEN1 gene have been described in sporadic gastrinomas and insulinomas. This study describes techniques used to identify mutations in the MEN1 gene in DNA extracted from paraffin-preserved tissue. Two novel mutations are identified in the MEN1 gene from nine archived paraffin-embedded neuroendocrine tumors, demonstrating that retrospective genetic analysis can be used to identify mutations in the MEN1 gene from preserved tissue. Conditions are provided by which paraffin-embedded tissue can be used as a source of genetic material for sequence information of sufficient quality for mutational studies of the MEN1 gene. It should also be possible to apply this retrospective genetic analysis of paraffin-embedded tissue to other disease models.

Original languageEnglish (US)
Pages (from-to)29-34
Number of pages6
JournalClinical chemistry
Volume45
Issue number1
Publication statusPublished - Jan 23 1999
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Mailman, M. D., Muscarella, P., Schirmer, W. J., Ellison, E. C., O'Dorisio, T. M., & Prior, T. W. (1999). Identification of MEN1 mutations in sporadic enteropancreatic neuroendocrine tumors by analysis of paraffin-embedded tissue. Clinical chemistry, 45(1), 29-34.