Identification of large rearrangements in cancer genomes with barcode linked reads

Li C. Xia, John M. Bell, Christina Wood-Bouwens, Jiamin J. Chen, Nancy R. Zhang, Hanlee P. Ji

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Large genomic rearrangements involve inversions, deletions and other structural changes that span Megabase segments of the human genome. This category of genetic aberration is the cause of many hereditary genetic disorders and contributes to pathogenesis of diseases like cancer. We developed a new algorithm called ZoomX for analysing barcode-linked sequence reads-these sequences can be traced to individual high molecular weight DNA molecules (<50 kb). To generate barcode linked sequence reads, we employ a library preparation technology (10X Genomics) that uses droplets to partition and barcode DNA molecules. Using linked read data from whole genome sequencing, we identify large genomic rearrangements, typically greater than 200kb, even when they are only present in low allelic fractions. Our algorithm uses a Poisson scan statistic to identify genomic rearrangement junctions, determine counts of junction-spanning molecules and calculate a Fisher's exact test for determining statistical significance for somatic aberrations. Utilizing a well-characterized human genome, we benchmarked this approach to accurately identify large rearrangement. Subsequently, we demonstrated that our algorithm identifies somatic rearrangements when present in lower allelic fractions as occurs in tumors. We characterized a set of complex cancer rearrangements with multiple classes of structural aberrations and with possible roles in oncogenesis.

Original languageEnglish (US)
Article numbere19
JournalNucleic acids research
Volume46
Issue number4
DOIs
StatePublished - Feb 28 2018
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Identification of large rearrangements in cancer genomes with barcode linked reads'. Together they form a unique fingerprint.

Cite this