Identification of in vivo phosphorylation sites for basic-directed kinases in murine mdr1b P-glycoprotein by combination of mass spectrometry and site-directed mutagenesis

J. S. Glavy; M. Wolfson; E. Nieves; E. K. Han; C. P.H. Yang; S. B. Horwitz; G. A. Orr

doi:10.1016/S0076-6879(98)92027-4

Identification of in vivo phosphorylation sites for basic-directed kinases in murine mdr1b P-glycoprotein by combination of mass spectrometry and site-directed mutagenesis

J. S. Glavy, M. Wolfson, E. Nieves, E. K. Han, C. P.H. Yang, S. B. Horwitz, G. A. Orr

Research output: Contribution to journal › Article › peer-review

Original language	English (US)
Pages (from-to)	342-358
Number of pages	17
Journal	Methods in enzymology
Volume	292
DOIs	https://doi.org/10.1016/S0076-6879(98)92027-4
State	Published - 1998
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/S0076-6879(98)92027-4

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@article{ce5466bff8334a2ca3ca7cb11b46f6f1,

title = "Identification of in vivo phosphorylation sites for basic-directed kinases in murine mdr1b P-glycoprotein by combination of mass spectrometry and site-directed mutagenesis",

author = "Glavy, {J. S.} and M. Wolfson and E. Nieves and Han, {E. K.} and Yang, {C. P.H.} and Horwitz, {S. B.} and Orr, {G. A.}",

note = "Funding Information: This work was supported in part by U.S. Public Health Service grants CA39821 (to S.B.H.), CA56677 (to G.A.O.), and 5P30 CA13330. J.S.G. was supported by National Institutes of Health Training Program in PharmacologicalS ciences grant 5T32 GM07260. Funding Information: I am indebted to Dr. J. F. Kuo for his support, encouragement, and interest throughout the course of this project. I thank Dr. Jan Pohl for sequence analysis, peptide synthesis, and invaluable advice on peptide purification. I thank Robert Raynor for providing PKC and Dr. Michael Gottesman for the KB-V1 cell line. This work was supported in part by grant CH-513 from the American Cancer Society.",

year = "1998",

doi = "10.1016/S0076-6879(98)92027-4",

language = "English (US)",

volume = "292",

pages = "342--358",

journal = "Methods in enzymology",

issn = "0076-6879",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Identification of in vivo phosphorylation sites for basic-directed kinases in murine mdr1b P-glycoprotein by combination of mass spectrometry and site-directed mutagenesis

AU - Glavy, J. S.

AU - Wolfson, M.

AU - Nieves, E.

AU - Han, E. K.

AU - Yang, C. P.H.

AU - Horwitz, S. B.

AU - Orr, G. A.

N1 - Funding Information: This work was supported in part by U.S. Public Health Service grants CA39821 (to S.B.H.), CA56677 (to G.A.O.), and 5P30 CA13330. J.S.G. was supported by National Institutes of Health Training Program in PharmacologicalS ciences grant 5T32 GM07260. Funding Information: I am indebted to Dr. J. F. Kuo for his support, encouragement, and interest throughout the course of this project. I thank Dr. Jan Pohl for sequence analysis, peptide synthesis, and invaluable advice on peptide purification. I thank Robert Raynor for providing PKC and Dr. Michael Gottesman for the KB-V1 cell line. This work was supported in part by grant CH-513 from the American Cancer Society.

PY - 1998

Y1 - 1998

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U2 - 10.1016/S0076-6879(98)92027-4

DO - 10.1016/S0076-6879(98)92027-4

M3 - Article

C2 - 9711566

AN - SCOPUS:0032323410

SN - 0076-6879

VL - 292

SP - 342

EP - 358

JO - Methods in enzymology

JF - Methods in enzymology

ER -

Identification of in vivo phosphorylation sites for basic-directed kinases in murine mdr1b P-glycoprotein by combination of mass spectrometry and site-directed mutagenesis

ASJC Scopus subject areas

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