Identification of GPCR localization in detergent resistant membranes

Ranju Kumari, Anna Francesconi

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Lipid domains of the plasma membrane were originally described as a cell matrix insoluble in cold nonionic detergents and enriched in glycosphingolipids. Because of these biochemical properties, these membrane domains were termed detergent-resistant membranes (DRMs) or detergent-insoluble glycolipid-enriched (DIG) membranes. Membrane rafts and caveolae are two types of lipid domains that share these properties, as well as structural/functional dependence on membrane cholesterol. Membrane rafts and caveolae are believed to act as signaling platforms for ligand-activated receptors, thereby contributing to the regulation of receptor function. Here we describe a simple method to assess the association of GPCRs with detergent resistant membranes in native brain tissue and cultured cells.

Original languageEnglish (US)
Pages (from-to)411-423
Number of pages13
JournalMethods in Molecular Biology
Volume746
DOIs
StatePublished - 2011

Keywords

  • Caveolae
  • DIG
  • DRM
  • Density gradients
  • Detergent resistant membranes
  • Flotation
  • GPCR
  • Lipid domains
  • Membrane rafts
  • mGluR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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