Identification of GPCR localization in detergent resistant membranes

Ranju Kumari, Anna Francesconi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Lipid domains of the plasma membrane were originally described as a cell matrix insoluble in cold nonionic detergents and enriched in glycosphingolipids. Because of these biochemical properties, these membrane domains were termed detergent-resistant membranes (DRMs) or detergent-insoluble glycolipid-enriched (DIG) membranes. Membrane rafts and caveolae are two types of lipid domains that share these properties, as well as structural/functional dependence on membrane cholesterol. Membrane rafts and caveolae are believed to act as signaling platforms for ligand-activated receptors, thereby contributing to the regulation of receptor function. Here we describe a simple method to assess the association of GPCRs with detergent resistant membranes in native brain tissue and cultured cells.

Original languageEnglish (US)
Pages (from-to)411-423
Number of pages13
JournalMethods in molecular biology (Clifton, N.J.)
Volume746
DOIs
StatePublished - 2011

Fingerprint

Detergents
Membranes
Caveolae
Lipids
Glycosphingolipids
Glycolipids
Cultured Cells
Cholesterol
Cell Membrane
Ligands
Brain

Keywords

  • Caveolae
  • Density gradients
  • Detergent resistant membranes
  • DIG
  • DRM
  • Flotation
  • GPCR
  • Lipid domains
  • Membrane rafts
  • mGluR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Identification of GPCR localization in detergent resistant membranes. / Kumari, Ranju; Francesconi, Anna.

In: Methods in molecular biology (Clifton, N.J.), Vol. 746, 2011, p. 411-423.

Research output: Contribution to journalArticle

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