Identification of G protein a-subunits in RINm5F cells and their selective interaction with galanin receptor

Mireille Cormont, Yannick Le Marchand-Brustel, Emmanuel Van Obberghen, Allen M. Spiegel, Geoffrey W G Sharp

Research output: Contribution to journalArticle

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Abstract

Galanin, an inhibitor of insulin secretion in pancreatic β-cells, exerts its multiple effects through mechanisms that are sensitive to pertussis toxin (PTX). G proteins have been characterized in RINm5F cells. By ADP ribosylation and immunoblotting, the α-subunits of Gi1, Gi2, Gi3, and two forms of Go were identified, Giα2 being predominant. As expected from a G protein-linked receptor, GTP and its nonhydrolyzable analogue GTP-Y-S decreased tracer galanin binding to cell membranes. This resulted from a change in receptor affinity without any modification in the number of sites. Selective antibodies against the COOH-terminal decapeptide of the á-subunits of the Gi and Go proteins were used to block G protein interaction before we studied galanin binding. Antibody AS, which selectively recognizes Giα1 and Giα2, decreased tracer galanin binding to membranes at concentrations where there were no effects of other antibodies specifically directed against Giα3 or Gαo. These data suggest that Gi1 and/or Gi2 interact with the galanin receptor and probably mediate the effects of galanin in pancreatic β-cells.

Original languageEnglish (US)
Pages (from-to)1170-1176
Number of pages7
JournalDiabetes
Volume40
Issue number9
StatePublished - Sep 1991
Externally publishedYes

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Galanin Receptors
Galanin
Protein Subunits
GTP-Binding Proteins
Guanosine Triphosphate
Antibodies
Pertussis Toxin
Immunoblotting
Adenosine Diphosphate
Cell Membrane
Insulin
Membranes
Proteins

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Cormont, M., Le Marchand-Brustel, Y., Van Obberghen, E., Spiegel, A. M., & Sharp, G. W. G. (1991). Identification of G protein a-subunits in RINm5F cells and their selective interaction with galanin receptor. Diabetes, 40(9), 1170-1176.

Identification of G protein a-subunits in RINm5F cells and their selective interaction with galanin receptor. / Cormont, Mireille; Le Marchand-Brustel, Yannick; Van Obberghen, Emmanuel; Spiegel, Allen M.; Sharp, Geoffrey W G.

In: Diabetes, Vol. 40, No. 9, 09.1991, p. 1170-1176.

Research output: Contribution to journalArticle

Cormont, M, Le Marchand-Brustel, Y, Van Obberghen, E, Spiegel, AM & Sharp, GWG 1991, 'Identification of G protein a-subunits in RINm5F cells and their selective interaction with galanin receptor', Diabetes, vol. 40, no. 9, pp. 1170-1176.
Cormont M, Le Marchand-Brustel Y, Van Obberghen E, Spiegel AM, Sharp GWG. Identification of G protein a-subunits in RINm5F cells and their selective interaction with galanin receptor. Diabetes. 1991 Sep;40(9):1170-1176.
Cormont, Mireille ; Le Marchand-Brustel, Yannick ; Van Obberghen, Emmanuel ; Spiegel, Allen M. ; Sharp, Geoffrey W G. / Identification of G protein a-subunits in RINm5F cells and their selective interaction with galanin receptor. In: Diabetes. 1991 ; Vol. 40, No. 9. pp. 1170-1176.
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