Identification of beta subunit of the rhesus monkey chorionic gonadotropin (rmCG)

Yun Chen, Zhe Liu, Bin Wang, Jing Pian Peng

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Chorionic gonadotropin (CG) is a placental derived hormone that plays a crucial role in successful implantation and establishment of early pregnancy in the primates. The rhesus monkey was chosen as a model to understand the feasibility of developing human DNA immuno-contraceptive. The coding region of rhesus monkey CG β-subunit (rmCGβ) was isolated by the TDRT-PCR method. The nucleotide sequence including the leader peptide was 499 nucleotide long and encoded 166 amino acids. In comparing with the previous known primates CG β-subunits, the rmCGβ was the highest degree of homology with baboon CG β-subunit at the deduced amino acid sequence (94%), 79.5% homology with human CG β-subunit and 70.4% homology with marmoset monkey CG β-subunit. The eukaryotic expression vector pCMV4-rmCGβ inserted full-coding cDNA sequence of rmCGβ was constructed, and the expression of rmCGβ-subunit in HeLa cells transient expressing system in vitro and BALB/c mice in vivo was determined. The results demonstrated that the recombinant PCMV4-rmCGβ eukaryotic expression vector could express rmCG β-subunit in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)157-163
Number of pages7
JournalMolecular and Cellular Biochemistry
Volume218
Issue number1-2
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

Keywords

  • DNA immuno-contraceptive
  • Expression
  • TDRT-PCR
  • rmCG β-subunit

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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