Identification of beta subunit of the rhesus monkey chorionic gonadotropin (rmCG)

Yun Chen, Z. Liu, B. Wang, J. P. Peng

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Chorionic gonadotropin (CG) is a placental derived hormone that plays a crucial role in successful implantation and establishment of early pregnancy in the primates. The rhesus monkey was chosen as a model to understand the feasibility of developing human DNA immuno-contraceptive. The coding region of rhesus monkey CG β-subunit (rmCGβ) was isolated by the TDRT-PCR method. The nucleotide sequence including the leader peptide was 499 nucleotide long and encoded 166 amino acids. In comparing with the previous known primates CG β-subunits, the rmCGβ was the highest degree of homology with baboon CG β-subunit at the deduced amino acid sequence (94%), 79.5% homology with human CG β-subunit and 70.4% homology with marmoset monkey CG β-subunit. The eukaryotic expression vector pCMV4-rmCGβ inserted full-coding cDNA sequence of rmCGβ was constructed, and the expression of rmCGβ-subunit in HeLa cells transient expressing system in vitro and BALB/c mice in vivo was determined. The results demonstrated that the recombinant PCMV4-rmCGβ eukaryotic expression vector could express rmCG β-subunit in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)157-163
Number of pages7
JournalMolecular and Cellular Biochemistry
Volume218
Issue number1-2
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Chorionic Gonadotropin
Macaca mulatta
Primates
Nucleotides
Placental Hormones
Amino Acids
Callithrix
Papio
Protein Sorting Signals
Contraceptive Agents
HeLa Cells
Haplorhini
Amino Acid Sequence
Complementary DNA
Hormones

Keywords

  • DNA immuno-contraceptive
  • Expression
  • rmCG β-subunit
  • TDRT-PCR

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Identification of beta subunit of the rhesus monkey chorionic gonadotropin (rmCG). / Chen, Yun; Liu, Z.; Wang, B.; Peng, J. P.

In: Molecular and Cellular Biochemistry, Vol. 218, No. 1-2, 2001, p. 157-163.

Research output: Contribution to journalArticle

Chen, Yun ; Liu, Z. ; Wang, B. ; Peng, J. P. / Identification of beta subunit of the rhesus monkey chorionic gonadotropin (rmCG). In: Molecular and Cellular Biochemistry. 2001 ; Vol. 218, No. 1-2. pp. 157-163.
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