Abstract
Chorionic gonadotropin (CG) is a placental derived hormone that plays a crucial role in successful implantation and establishment of early pregnancy in the primates. The rhesus monkey was chosen as a model to understand the feasibility of developing human DNA immuno-contraceptive. The coding region of rhesus monkey CG β-subunit (rmCGβ) was isolated by the TDRT-PCR method. The nucleotide sequence including the leader peptide was 499 nucleotide long and encoded 166 amino acids. In comparing with the previous known primates CG β-subunits, the rmCGβ was the highest degree of homology with baboon CG β-subunit at the deduced amino acid sequence (94%), 79.5% homology with human CG β-subunit and 70.4% homology with marmoset monkey CG β-subunit. The eukaryotic expression vector pCMV4-rmCGβ inserted full-coding cDNA sequence of rmCGβ was constructed, and the expression of rmCGβ-subunit in HeLa cells transient expressing system in vitro and BALB/c mice in vivo was determined. The results demonstrated that the recombinant PCMV4-rmCGβ eukaryotic expression vector could express rmCG β-subunit in vitro and in vivo.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 157-163 |
| Number of pages | 7 |
| Journal | Molecular and Cellular Biochemistry |
| Volume | 218 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - 2001 |
| Externally published | Yes |
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Keywords
- DNA immuno-contraceptive
- Expression
- rmCG β-subunit
- TDRT-PCR
ASJC Scopus subject areas
- Clinical Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
Cite this
Identification of beta subunit of the rhesus monkey chorionic gonadotropin (rmCG). / Chen, Yun; Liu, Z.; Wang, B.; Peng, J. P.
In: Molecular and Cellular Biochemistry, Vol. 218, No. 1-2, 2001, p. 157-163.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of beta subunit of the rhesus monkey chorionic gonadotropin (rmCG)
AU - Chen, Yun
AU - Liu, Z.
AU - Wang, B.
AU - Peng, J. P.
PY - 2001
Y1 - 2001
N2 - Chorionic gonadotropin (CG) is a placental derived hormone that plays a crucial role in successful implantation and establishment of early pregnancy in the primates. The rhesus monkey was chosen as a model to understand the feasibility of developing human DNA immuno-contraceptive. The coding region of rhesus monkey CG β-subunit (rmCGβ) was isolated by the TDRT-PCR method. The nucleotide sequence including the leader peptide was 499 nucleotide long and encoded 166 amino acids. In comparing with the previous known primates CG β-subunits, the rmCGβ was the highest degree of homology with baboon CG β-subunit at the deduced amino acid sequence (94%), 79.5% homology with human CG β-subunit and 70.4% homology with marmoset monkey CG β-subunit. The eukaryotic expression vector pCMV4-rmCGβ inserted full-coding cDNA sequence of rmCGβ was constructed, and the expression of rmCGβ-subunit in HeLa cells transient expressing system in vitro and BALB/c mice in vivo was determined. The results demonstrated that the recombinant PCMV4-rmCGβ eukaryotic expression vector could express rmCG β-subunit in vitro and in vivo.
AB - Chorionic gonadotropin (CG) is a placental derived hormone that plays a crucial role in successful implantation and establishment of early pregnancy in the primates. The rhesus monkey was chosen as a model to understand the feasibility of developing human DNA immuno-contraceptive. The coding region of rhesus monkey CG β-subunit (rmCGβ) was isolated by the TDRT-PCR method. The nucleotide sequence including the leader peptide was 499 nucleotide long and encoded 166 amino acids. In comparing with the previous known primates CG β-subunits, the rmCGβ was the highest degree of homology with baboon CG β-subunit at the deduced amino acid sequence (94%), 79.5% homology with human CG β-subunit and 70.4% homology with marmoset monkey CG β-subunit. The eukaryotic expression vector pCMV4-rmCGβ inserted full-coding cDNA sequence of rmCGβ was constructed, and the expression of rmCGβ-subunit in HeLa cells transient expressing system in vitro and BALB/c mice in vivo was determined. The results demonstrated that the recombinant PCMV4-rmCGβ eukaryotic expression vector could express rmCG β-subunit in vitro and in vivo.
KW - DNA immuno-contraceptive
KW - Expression
KW - rmCG β-subunit
KW - TDRT-PCR
UR - http://www.scopus.com/inward/record.url?scp=0035071411&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035071411&partnerID=8YFLogxK
U2 - 10.1023/A:1007233329355
DO - 10.1023/A:1007233329355
M3 - Article
C2 - 11330831
AN - SCOPUS:0035071411
VL - 218
SP - 157
EP - 163
JO - Molecular and Cellular Biochemistry
JF - Molecular and Cellular Biochemistry
SN - 0300-8177
IS - 1-2
ER -