Identification of an essential cis-element near the transcription start site for transcriptional activation of the proliferating cell nuclear antigen gene

Dan Yang Huang, Michael B. Prystowsky

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Interleukin 2 (IL-2) stimulates T lymphocyte proliferation and induces the expression of proliferating cell nuclear antigen (PCNA), a processivity factor for DNA polymerase δ. Previously, deletion analysis suggested cis- element(s) in the proximal region of the PCNA promoter (-40 to +143) are required for IL-2 induction in cloned T lymphocytes. The sequence 5'- TTGCGGGC-3' located at +10 to +17 is similar to the E2F consensus binding site and is required for optimal PCNA promoter activity. In IL-2-stimulated T cells, nuclear proteins are induced to bind to this sequence as demonstrated using electrophoretic mobility shift assay (EMSA), competition EMSA, and methylation interference analysis. A 180-kDa polypeptide was detected by UV cross-linking to bind specifically to the PCNA E2F-like sequence. Our data indicate that the protein bound to the PCNA E2F-like site is not one of the transcription factor E2F proteins. Our results demonstrate that the E2F-like sequence and the protein(s) binding to it are required for optimal PCNA promoter activity and IL-2 induction of PCNA expression.

Original languageEnglish (US)
Pages (from-to)1218-1225
Number of pages8
JournalJournal of Biological Chemistry
Volume271
Issue number2
DOIs
Publication statusPublished - Jan 12 1996

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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