Identification of a thymidylate synthase ribonucleoprotein complex in human colon cancer cells

E. Chu, D. M. Voeller, K. L. Jones, T. Takechi, G. F. Maley, F. Maley, S. Segal, C. J. Allegra

Research output: Contribution to journalArticlepeer-review

81 Scopus citations

Abstract

Translation of thymidylate synthase (TS) mRNA is controlled by its own protein product, TS, in an autoregulatory manner. Direct binding of TS protein to two different cis-acting elements on the TS mRNA is associated with this translational regulation. In this study, an immunoprecipitation- reverse transcription-PCR technique was used to identify a TS ribonucleoprotein (RNP) complex in cultured human colon cancer cells. Using antibodies specific for TS protein, we show that TS is complexed in vivo with its own TS RNA. Furthermore, evidence demonstrating a direct interaction between the mRNA of the nuclear oncogene c-myc and TS protein is presented.

Original languageEnglish (US)
Pages (from-to)207-213
Number of pages7
JournalMolecular and cellular biology
Volume14
Issue number1
DOIs
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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