Identification of a thymidylate synthase ribonucleoprotein complex in human colon cancer cells

Edward Chu; Donna M. Voeller; Kristen L. Jones; Teiji Takechi; Gladys F. Maley; Frank Maley; Shoshana Segal; Carmen J. Allegra

Identification of a thymidylate synthase ribonucleoprotein complex in human colon cancer cells

Edward Chu, Donna M. Voeller, Kristen L. Jones, Teiji Takechi, Gladys F. Maley, Frank Maley, Shoshana Segal, Carmen J. Allegra

Research output: Contribution to journal › Article › peer-review

84 Scopus citations

Abstract

Translation of thymidylate synthase (TS) mRNA is controlled by its own protein product, TS, in an autoregulatory manner. Direct binding of TS protein to two different cis-acting elements on the TS mRNA is associated with this translational regulation. In this study, an immunoprecipitation-reverse transcription-PCR technique was used to identify a TS ribonucleoprotein (RNP) complex in cultured human colon cancer cells. Using antibodies specific for TS protein, we show that TS is complexed in vivo with its own TS RNA. Furthermore, evidence demonstrating a direct interaction between the mRNA of the nuclear oncogene c-myc and TS protein is presented.

Original language	English (US)
Pages (from-to)	207-213
Number of pages	7
Journal	Molecular and cellular biology
Volume	14
Issue number	1
State	Published - 1994
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

@article{10ba5976b6ca4f539dd59af42029b689,

title = "Identification of a thymidylate synthase ribonucleoprotein complex in human colon cancer cells",

abstract = "Translation of thymidylate synthase (TS) mRNA is controlled by its own protein product, TS, in an autoregulatory manner. Direct binding of TS protein to two different cis-acting elements on the TS mRNA is associated with this translational regulation. In this study, an immunoprecipitation-reverse transcription-PCR technique was used to identify a TS ribonucleoprotein (RNP) complex in cultured human colon cancer cells. Using antibodies specific for TS protein, we show that TS is complexed in vivo with its own TS RNA. Furthermore, evidence demonstrating a direct interaction between the mRNA of the nuclear oncogene c-myc and TS protein is presented.",

author = "Edward Chu and Voeller, {Donna M.} and Jones, {Kristen L.} and Teiji Takechi and Maley, {Gladys F.} and Frank Maley and Shoshana Segal and Allegra, {Carmen J.}",

year = "1994",

language = "English (US)",

volume = "14",

pages = "207--213",

journal = "Molecular and cellular biology",

issn = "0270-7306",

publisher = "American Society for Microbiology",

number = "1",

}

TY - JOUR

T1 - Identification of a thymidylate synthase ribonucleoprotein complex in human colon cancer cells

AU - Chu, Edward

AU - Voeller, Donna M.

AU - Jones, Kristen L.

AU - Takechi, Teiji

AU - Maley, Gladys F.

AU - Maley, Frank

AU - Segal, Shoshana

AU - Allegra, Carmen J.

PY - 1994

Y1 - 1994

N2 - Translation of thymidylate synthase (TS) mRNA is controlled by its own protein product, TS, in an autoregulatory manner. Direct binding of TS protein to two different cis-acting elements on the TS mRNA is associated with this translational regulation. In this study, an immunoprecipitation-reverse transcription-PCR technique was used to identify a TS ribonucleoprotein (RNP) complex in cultured human colon cancer cells. Using antibodies specific for TS protein, we show that TS is complexed in vivo with its own TS RNA. Furthermore, evidence demonstrating a direct interaction between the mRNA of the nuclear oncogene c-myc and TS protein is presented.

AB - Translation of thymidylate synthase (TS) mRNA is controlled by its own protein product, TS, in an autoregulatory manner. Direct binding of TS protein to two different cis-acting elements on the TS mRNA is associated with this translational regulation. In this study, an immunoprecipitation-reverse transcription-PCR technique was used to identify a TS ribonucleoprotein (RNP) complex in cultured human colon cancer cells. Using antibodies specific for TS protein, we show that TS is complexed in vivo with its own TS RNA. Furthermore, evidence demonstrating a direct interaction between the mRNA of the nuclear oncogene c-myc and TS protein is presented.

UR - http://www.scopus.com/inward/record.url?scp=0028031680&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028031680&partnerID=8YFLogxK

M3 - Article

C2 - 8264588

AN - SCOPUS:0028031680

SN - 0270-7306

VL - 14

SP - 207

EP - 213

JO - Molecular and cellular biology

JF - Molecular and cellular biology

IS - 1

ER -

Identification of a thymidylate synthase ribonucleoprotein complex in human colon cancer cells

Abstract

ASJC Scopus subject areas

UN SDGs

Other files and links

Fingerprint

Cite this