Identification of a sequence element on the 3' slide of AAUAAA which is necessary for simian virus 40 late mrna 3'-end processing

Moshe J. Sadofsky, S. Connelly, J. L. Manley, J. C. Alwine

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Our previous studies of the 3'-end processing of simian virus 40 late mRNAs indicated the existence of an essential element (or elements) downstream of the AAUAAA signal. We report here the use of transient expression analysis to study a functional element which we located within the sequence AGGUUUUUU, beginning 59 nucleotides downstream of the recognized signal AAUAAA. Deletion of this element resulted in at least a 75% drop in 3'-end processing at the normal site and appearance of readthrough transcripts with alternate 3' ends. Some flexibility in the downstream position of this element relative to the AAUAAA was noted by deletion analysis. Using computer sequence comparison, we located homologous regions within downstream sequences of other genes, suggesting a generalized sequence element. In addition, specific complementarity is noted between the downstream element and U4 RNA. The possibility that this complementarity could participate in 3'-end site selection is discussed.

Original languageEnglish (US)
Pages (from-to)2713-2719
Number of pages7
JournalMolecular and Cellular Biology
Volume5
Issue number10
StatePublished - 1985
Externally publishedYes

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Simian virus 40
Nucleotides
Messenger RNA
Genes
U4 small nuclear RNA

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Identification of a sequence element on the 3' slide of AAUAAA which is necessary for simian virus 40 late mrna 3'-end processing. / Sadofsky, Moshe J.; Connelly, S.; Manley, J. L.; Alwine, J. C.

In: Molecular and Cellular Biology, Vol. 5, No. 10, 1985, p. 2713-2719.

Research output: Contribution to journalArticle

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