Identification of a new spore wall protein from Encephalitozoon cuniculi

Yanji Xu, Peter Takvorian, Ann Cali, Fang Wang, Hong Zhang, George Orr, Louis M. Weiss

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Microsporidia form environmentally resistant spores that are critical for their host-to-host transmission and persistence in the environment. The spore walls of these organisms are composed of two layers, the exospore and the endospore. Two spore wall proteins (SWP1 and SWP2) have been previously identified in members of the Encephalitozoonidae family. These proteins localize to the exospore. The endospore is known to contain chitin, and a putative glycosylphosphatidylinositol (GPI)-anchored chitin deacetylase has been localized to the plasmalemma-endospore interface. Using proteomic techniques, we have identified a new spore wall protein (SWP3) that is located in the endospore. The gene for this protein is located on chromosome 1 and corresponds to the open reading frame ECU01_1270. SWP3 is predicted to have a signal peptide and to be GPI anchored. Consistent with these modifications, two-dimensional electrophoresis demonstrated that SWP3 has an acidic pI and a molecular mass of <20 kDa. By immunoelectron microscopy, this protein was found on the cell surface during sporogony and in the endospore in mature spores. SWP3 has several potential O-glycosylation sites, and it is possible that it is a mannosylated protein like the major polar tube protein (PTP1).

Original languageEnglish (US)
Pages (from-to)239-247
Number of pages9
JournalInfection and Immunity
Volume74
Issue number1
DOIs
StatePublished - Jan 2006

Fingerprint

Encephalitozoon cuniculi
Spores
Proteins
Glycosylphosphatidylinositols
chitin deacetylase
Microsporidia
Chitin
Immunoelectron Microscopy
Chromosomes, Human, Pair 1
Protein Sorting Signals
Glycosylation
Proteomics
Open Reading Frames
Electrophoresis

ASJC Scopus subject areas

  • Immunology

Cite this

Identification of a new spore wall protein from Encephalitozoon cuniculi. / Xu, Yanji; Takvorian, Peter; Cali, Ann; Wang, Fang; Zhang, Hong; Orr, George; Weiss, Louis M.

In: Infection and Immunity, Vol. 74, No. 1, 01.2006, p. 239-247.

Research output: Contribution to journalArticle

Xu, Yanji ; Takvorian, Peter ; Cali, Ann ; Wang, Fang ; Zhang, Hong ; Orr, George ; Weiss, Louis M. / Identification of a new spore wall protein from Encephalitozoon cuniculi. In: Infection and Immunity. 2006 ; Vol. 74, No. 1. pp. 239-247.
@article{9fb618c4d76c4fe383bb752135a2de19,
title = "Identification of a new spore wall protein from Encephalitozoon cuniculi",
abstract = "Microsporidia form environmentally resistant spores that are critical for their host-to-host transmission and persistence in the environment. The spore walls of these organisms are composed of two layers, the exospore and the endospore. Two spore wall proteins (SWP1 and SWP2) have been previously identified in members of the Encephalitozoonidae family. These proteins localize to the exospore. The endospore is known to contain chitin, and a putative glycosylphosphatidylinositol (GPI)-anchored chitin deacetylase has been localized to the plasmalemma-endospore interface. Using proteomic techniques, we have identified a new spore wall protein (SWP3) that is located in the endospore. The gene for this protein is located on chromosome 1 and corresponds to the open reading frame ECU01_1270. SWP3 is predicted to have a signal peptide and to be GPI anchored. Consistent with these modifications, two-dimensional electrophoresis demonstrated that SWP3 has an acidic pI and a molecular mass of <20 kDa. By immunoelectron microscopy, this protein was found on the cell surface during sporogony and in the endospore in mature spores. SWP3 has several potential O-glycosylation sites, and it is possible that it is a mannosylated protein like the major polar tube protein (PTP1).",
author = "Yanji Xu and Peter Takvorian and Ann Cali and Fang Wang and Hong Zhang and George Orr and Weiss, {Louis M.}",
year = "2006",
month = "1",
doi = "10.1128/IAI.74.1.239-247.2006",
language = "English (US)",
volume = "74",
pages = "239--247",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Identification of a new spore wall protein from Encephalitozoon cuniculi

AU - Xu, Yanji

AU - Takvorian, Peter

AU - Cali, Ann

AU - Wang, Fang

AU - Zhang, Hong

AU - Orr, George

AU - Weiss, Louis M.

PY - 2006/1

Y1 - 2006/1

N2 - Microsporidia form environmentally resistant spores that are critical for their host-to-host transmission and persistence in the environment. The spore walls of these organisms are composed of two layers, the exospore and the endospore. Two spore wall proteins (SWP1 and SWP2) have been previously identified in members of the Encephalitozoonidae family. These proteins localize to the exospore. The endospore is known to contain chitin, and a putative glycosylphosphatidylinositol (GPI)-anchored chitin deacetylase has been localized to the plasmalemma-endospore interface. Using proteomic techniques, we have identified a new spore wall protein (SWP3) that is located in the endospore. The gene for this protein is located on chromosome 1 and corresponds to the open reading frame ECU01_1270. SWP3 is predicted to have a signal peptide and to be GPI anchored. Consistent with these modifications, two-dimensional electrophoresis demonstrated that SWP3 has an acidic pI and a molecular mass of <20 kDa. By immunoelectron microscopy, this protein was found on the cell surface during sporogony and in the endospore in mature spores. SWP3 has several potential O-glycosylation sites, and it is possible that it is a mannosylated protein like the major polar tube protein (PTP1).

AB - Microsporidia form environmentally resistant spores that are critical for their host-to-host transmission and persistence in the environment. The spore walls of these organisms are composed of two layers, the exospore and the endospore. Two spore wall proteins (SWP1 and SWP2) have been previously identified in members of the Encephalitozoonidae family. These proteins localize to the exospore. The endospore is known to contain chitin, and a putative glycosylphosphatidylinositol (GPI)-anchored chitin deacetylase has been localized to the plasmalemma-endospore interface. Using proteomic techniques, we have identified a new spore wall protein (SWP3) that is located in the endospore. The gene for this protein is located on chromosome 1 and corresponds to the open reading frame ECU01_1270. SWP3 is predicted to have a signal peptide and to be GPI anchored. Consistent with these modifications, two-dimensional electrophoresis demonstrated that SWP3 has an acidic pI and a molecular mass of <20 kDa. By immunoelectron microscopy, this protein was found on the cell surface during sporogony and in the endospore in mature spores. SWP3 has several potential O-glycosylation sites, and it is possible that it is a mannosylated protein like the major polar tube protein (PTP1).

UR - http://www.scopus.com/inward/record.url?scp=29644438012&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29644438012&partnerID=8YFLogxK

U2 - 10.1128/IAI.74.1.239-247.2006

DO - 10.1128/IAI.74.1.239-247.2006

M3 - Article

C2 - 16368977

AN - SCOPUS:29644438012

VL - 74

SP - 239

EP - 247

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 1

ER -