Identification of a mammalian nuclear factor and human cDNA-encoded proteins that recognize DNA containing apurinic sites

Jack Lenz, Sharon A. Okenquist, Joseph E. Losardo, Krista K. Hamilton, Paul W. Doetsch

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Damage to DNA can have lethal or mutagenic consequences for cells unless it is detected and repaired by cellular proteins. Repair depends on the ability of cellular factors to distinguish the damaged sites. Electrophoretic binding assays were used to identify a factor from the nuclei of mammalian cells that bound to DNA containing apurinic sites. A binding assay based on the use of β-galactosidase fusion proteins was subsequently used to isolate recombinant clones of human cDNAs that encoded apurinic DNA-binding proteins. Two distinct human cDNAs were identified that encoded proteins that bound apurinic DNA preferentially over undamaged, methylated, or UV-irradiated DNA. These approaches may offer a general method for the detection of proteins that recognize various types of DNA damage and for the cloning of genes encoding such proteins.

Original languageEnglish (US)
Pages (from-to)3396-3400
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number9
StatePublished - May 1990

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Complementary DNA
DNA
Proteins
DNA Damage
Galactosidases
DNA-Binding Proteins
Cell Nucleus
Organism Cloning
Clone Cells
Genes

Keywords

  • λgt11
  • DNA repair
  • DNA-binding proteins

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Identification of a mammalian nuclear factor and human cDNA-encoded proteins that recognize DNA containing apurinic sites. / Lenz, Jack; Okenquist, Sharon A.; Losardo, Joseph E.; Hamilton, Krista K.; Doetsch, Paul W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 9, 05.1990, p. 3396-3400.

Research output: Contribution to journalArticle

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