The purpose of this work was to develop a system to identify virulence determinants of M. tuberculosis by genetic complementation. The ability to grow in mouse spleen and/or lung was defined as a potential phenotype for virulence. Enrichment for growing recombinant clones from a pool of H37Ra transformants containing the integrating pYUB178::H37Rv cosmid library was accomplished by in vivo selection. A molecular strategy was devised to isolate and clone the 25-kb 1137Rv genomic fragment ivg that conferred in vivo growth advantage to H37Ra. This study is a first step toward understanding the genetics of virulence in M. tuberculosis. A detailed discription of these experiments has been submitted for publication in Infection and Immunity.
|Original language||English (US)|
|Number of pages||3|
|Journal||Infectious Agents and Disease|
|Publication status||Published - Dec 1 1993|
ASJC Scopus subject areas
- Microbiology (medical)