Identification of a genomic fragment of Mycobacterium tuberculosis responsible for in vivo growth advantage

L. Pascopella, F. M. Collins, J. M. Martin, W. R. Jacobs, B. R. Bloom

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The purpose of this work was to develop a system to identify virulence determinants of M. tuberculosis by genetic complementation. The ability to grow in mouse spleen and/or lung was defined as a potential phenotype for virulence. Enrichment for growing recombinant clones from a pool of H37Ra transformants containing the integrating pYUB178::H37Rv cosmid library was accomplished by in vivo selection. A molecular strategy was devised to isolate and clone the 25-kb 1137Rv genomic fragment ivg that conferred in vivo growth advantage to H37Ra. This study is a first step toward understanding the genetics of virulence in M. tuberculosis. A detailed discription of these experiments has been submitted for publication in Infection and Immunity.

Original languageEnglish (US)
Pages (from-to)282-284
Number of pages3
JournalInfectious Agents and Disease
Volume2
Issue number4
StatePublished - Dec 1 1993

ASJC Scopus subject areas

  • Microbiology (medical)

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